Determination of Colchicine in Pharmaceutical Formulations and Urine by Multiple-Pulse Amperometric Detection in an FIA System Using Boron-Doped Diamond Electrode

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Abstract

This work presents a simple and fast method for colchicine (CO) determination in pharmaceutical formulations and urine by multiple pulse amperometry (MPA) with flow injection analysis (FIA) system using a boron-doped diamond electrode. In optimized conditions, it was possible to quantify CO in urine samples without interference of uric acid and ascorbic acid. The working linear range for CO quantification was achieved from 1.0 × 10-7 to 0.5 × 10-3mol L-1 with a low limit of detection of 2.14 × 10-8mol L-1. Furthermore, the proposed method showed high repeatability for 10 consecutive injections of 1.0 × 10-4mol L-1 CO (relative standard deviation = 1.28%) and good analytical frequency (30 determinations per hour). The addition and recovery studies in all pharmaceutical samples were approximately 100% and the results for CO determination were compared by high performance liquid chromatography (HPLC) with UV detection.

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Author and article information

Contributors

Débora A. R. Moreira: Role: ND

Fernando M. de Oliveira: Role: ND

Dilton M. Pimentel: Role: ND

Tiago J. Guedes: Role: ND

Rita C. S. Luz: Role: ND

Flávio S. Damos: Role: ND

Arnaldo C. Pereira: Role: ND

Rodrigo A. B. da Silva: Role: ND

Wallans T. P. dos Santos: Role: ND

Journal

Journal ID (publisher-id): jbchs

Title: Journal of the Brazilian Chemical Society

Abbreviated Title: J. Braz. Chem. Soc.

Publisher: Sociedade Brasileira de Química (São Paulo, SP, Brazil )

ISSN (Print): 0103-5053

ISSN (Electronic): 1678-4790

Publication date (Print and electronic): September 2018

Volume: 29

Issue: 9

Pages: 1796-1802

Affiliations

[6] Diamantina Minas Gerais orgnameUniversidade Federal dos Vales do Jequitinhonha e Mucuri orgdiv1Departamento de Farmácia Brazil

[1] São João Del Minas Gerais orgnameUniversidade Federal de São João del-Rei orgdiv1Departamento de Ciências Naturais Brazil

[2] Diamantina Minas Gerais orgnameUniversidade Federal dos Vales do Jequitinhonha e Mucuri orgdiv1Departamento de Química Brazil

[4] São Luís orgnameUniversidade Federal do Maranhão orgdiv1Departamento de Química Brazil

[5] Monte Carmelo Minas Gerais orgnameUniversidade Federal de Uberlândia orgdiv1Instituto de Química Brazil

[3] Betim Minas Gerais orgnameInstituto Federal de Minas Gerais Brazil

Article

Publisher ID: S0103-50532018000901796

DOI: 10.21577/0103-5053.20180055

SO-VID: 33f969f0-00c4-4493-a73e-c4ec2bcdd435

License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

History

Date accepted : 20 March 2018

Date received : 10 January 2018

Page count

Figures: 0, Tables: 0, Equations: 0, References: 51, Pages: 7

Product

SciELO Brazil

Keywords: flow-injection analysis,colchicine,boron-doped diamond,multiple pulse amperometry

Data availability:

Keywords: flow-injection analysis, colchicine, boron-doped diamond, multiple pulse amperometry

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