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      In vivo Assessment of Lens Transmission for Blue-Green Light by Autofluorescence Measurement

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          Abstract

          A simple quantitative and reproducible method for evaluating lens transmission (λ = 490 and 530 nm) is described. It is based on the measurement of autofluorescence in the anterior and the posterior part of the lens by means of a fluorophotometer, assuming an about equal fluorescence quantum efficiency in both parts. Consequently any difference in fluorescence between both parts can be attributed to a loss of exciting and fluorescent light in the lens. For both wavelengths, the average lens transmission is determined by this method in a normal population as a function of age.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1985
          1985
          04 December 2009
          : 17
          : 2
          : 90-95
          Affiliations
          Department of Ophthalmology, Leiden University Medical Centre, Leiden, The Netherlands
          Article
          265356 Ophthalmic Res 1985;17:90–95
          10.1159/000265356
          3982789
          © 1985 S. Karger AG, Basel

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          Pages: 6
          Categories
          Original Paper

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