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      Studies on metabolism of total glucosides of paeony from Paeoniae Radix Alba in rats by UPLC-Q-TOF-MS/MS.

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          Abstract

          Total glucosides of paeony are the active constituents of Paeoniae Radix Alba. In this study, a novel strategy was proposed to find more metabolites and the differences between paeoniflorin, albiflorin and total glucosides of paeony (TGP). This strategy was characterized as follows: firstly, the animals were divided into three groups (paeoniflorin, albiflorin and TGP) to identify the source of TGP metabolites from paeoniflorin or albiflorin; secondly, a generic information-dependent acquisition scan for the low-level metabolites was triggered by the multiple mass defect filter and dynamic background subtraction; thirdly, the metabolites were identified with a combination of data-processing methods including mass defect filtering, neutral loss filtering and product ion filtering; finally, a comparative study was used in the metabolism of paeoniflorin, albiflorin and TGP. Based on the strategy, 18 metabolites of TGP, 10 metabolites of paeoniflorin and 13 metabolites of albiflorin were identified respectively. The results indicated that the hydrolysis, conjugation reaction and oxidization were the major metabolic pathways, and the metabolic sites were the glycosidic linkage, the ester bond and the benzene ring. This study is first to explore the metabolism of TGP, and these findings enhance our understanding of the metabolism and the interactions of paeoniflrin and albiflorin in TGP.

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          Author and article information

          Journal
          Biomed. Chromatogr.
          Biomedical chromatography : BMC
          Wiley-Blackwell
          1099-0801
          0269-3879
          Nov 2015
          : 29
          : 11
          Affiliations
          [1 ] Pharmacology Laboratory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.
          [2 ] Shijiazhuang City Hospital of Traditional Chinese Medicine, Shijiazhuang, 050091, China.
          [3 ] Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, 050035, China.
          Article
          10.1002/bmc.3493
          26058364
          34c83dba-37e8-454b-9d78-dc324bce0b79
          History

          identification,UPLC-Q-TOF-MS/MS,albiflorin,paeoniflorin,total glucosides of paeony

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