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      Binding of Dopamine to Bovine Anterior Pituitary Gland Membranes

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          Abstract

          [<sup>3</sup>H]-dopamine (DA) binding to bovine anterior pituitary membranes was measured using sensitive in vitro ultrafiltration and centrifugation techniques. The specific interaction of [<sup>3</sup>H]-DA with the membrane fraction reached a steady-state level within 15 min at 30°C and was reversible by incubating with excess nonradioactive DA. Scatchard analysis suggests the presence of 2 sites for DA specific binding having K < j values of 4.4 × 10<sup>–10</sup> M and 4.7 × 10<sup>–8</sup> M, respectively. Correspondingly, the total receptor concentrations were calculated to be 336 and 2,340 pmoles/g protein. Blockade of [<sup>3</sup>H]-DA binding was produced most effectively by ergocryptine and apomorphine. Perphenazine and haloperidol were considerably less active and pimozide did not compete. The importance of DA in the regulation of prolactin (Prl) secretion and the characterization of pituitary DA receptor sites are discussed.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1978
          1978
          25 March 2008
          : 25
          : 1
          : 32-46
          Affiliations
          Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Virginia School of Medicine Charlottesville, Va.
          Article
          122762 Neuroendocrinology 1978;25:32–46
          10.1159/000122762
          652118
          © 1978 S. Karger AG, Basel

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          Page count
          Pages: 15
          Categories
          Original Paper

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