Binding of Dopamine to Bovine Anterior Pituitary Gland Membranes

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Abstract

[3H]-dopamine (DA) binding to bovine anterior pituitary membranes was measured using sensitive in vitro ultrafiltration and centrifugation techniques. The specific interaction of [3H]-DA with the membrane fraction reached a steady-state level within 15 min at 30°C and was reversible by incubating with excess nonradioactive DA. Scatchard analysis suggests the presence of 2 sites for DA specific binding having K < j values of 4.4 × 10–10 M and 4.7 × 10–8 M, respectively. Correspondingly, the total receptor concentrations were calculated to be 336 and 2,340 pmoles/g protein. Blockade of [3H]-DA binding was produced most effectively by ergocryptine and apomorphine. Perphenazine and haloperidol were considerably less active and pimozide did not compete. The importance of DA in the regulation of prolactin (Prl) secretion and the characterization of pituitary DA receptor sites are discussed.

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Author and article information

Journal

Journal ID (publisher-id): NEN

Journal ID (nlm-ta): Neuroendocrinology

Journal ID (doi): 10.1159/issn.0028-3835

Title: Neuroendocrinology

Publisher: S. Karger AG

ISSN (Print): 0028-3835

ISSN (Electronic): 1423-0194

Publication date Subscription-year: 1978

Publication date (Print): 1978

Publication date (Electronic): 25 March 2008

Volume: 25

Issue: 1

Pages: 32-46

Affiliations

Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Virginia School of Medicine Charlottesville, Va.

Article

Publisher ID: 122762 Other ID: Neuroendocrinology 1978;25:32–46

DOI: 10.1159/000122762

PubMed ID: 652118

SO-VID: 34c979ed-9139-4def-b39c-0a7be83fb1f9

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 06 May 1977

Date accepted : 09 August 1977

Page count

Pages: 15

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