Elisa Laurenti 1 , 8 , 9 , ∗ , Catherine Frelin 1 , 8 , Stephanie Xie 1 , 8 , Robin Ferrari 1 , 3 , Cyrille F. Dunant 4 , Sasan Zandi 1 , Andrea Neumann 1 , Ian Plumb 1 , Sergei Doulatov 5 , Jing Chen 6 , Craig April 6 , Jian-Bing Fan 6 , Norman Iscove 1 , 7 , John E. Dick 1 , 2 , ∗∗
05 March 2015
Regulated blood production is achieved through the hierarchical organization of dormant hematopoietic stem cell (HSC) subsets that differ in self-renewal potential and division frequency, with long-term (LT)-HSCs dividing the least. The molecular mechanisms underlying this variability in HSC division kinetics are unknown. We report here that quiescence exit kinetics are differentially regulated within human HSC subsets through the expression level of CDK6. LT-HSCs lack CDK6 protein. Short-term (ST)-HSCs are also quiescent but contain high CDK6 protein levels that permit rapid cell cycle entry upon mitogenic stimulation. Enforced CDK6 expression in LT-HSCs shortens quiescence exit and confers competitive advantage without impacting function. Computational modeling suggests that this independent control of quiescence exit kinetics inherently limits LT-HSC divisions and preserves the HSC pool to ensure lifelong hematopoiesis. Thus, differential expression of CDK6 underlies heterogeneity in stem cell quiescence states that functionally regulates this highly regenerative system.
The hematopoietic stem cell (HSC) compartment is heterogeneous in terms of cell cycle properties. Laurenti et al. show that the timing of exit from quiescence in human HSC subsets is controlled by CDK6 expression levels. This differential control has an impact on the long-term preservation of the HSC pool.