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      Biomolecular Deuteration for Neutron Structural Biology and Dynamics.

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          Abstract

          Neutron scattering studies provide important information in structural biology that is not accessible using other approaches. The uniqueness of the technique, and its complementarity with X-ray scattering, is greatest when full use is made of deuterium labeling. The ability to produce tailor-made deuterium-labeled biological macromolecules allows neutron studies involving solution scattering, crystallography, reflection, and dynamics to be optimized in a manner that has major impact on the scope, quality, and throughput of work in these areas. Deuteration facilities have now been developed at many neutron centres throughout the world; these are having a crucial effect on neutron studies in the life sciences and on biologically related studies in soft matter. This chapter describes methods that have been developed for the efficient production of deuterium-labeled samples for a wide range of neutron scattering applications. Examples are given that illustrate the use of these samples for each of the main techniques. Perspectives for biological deuterium labeling are discussed in relation to developments at current facilities and those that are planned in the future.

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          Author and article information

          Journal
          Meth. Enzymol.
          Methods in enzymology
          1557-7988
          0076-6879
          2016
          : 566
          Affiliations
          [1 ] Life Sciences Group, Institut Laue-Langevin, Grenoble Cedex 9, France.
          [2 ] Life Sciences Group, Institut Laue-Langevin, Grenoble Cedex 9, France; Department of Structural and Molecular Biology, University College London, London, United Kingdom.
          [3 ] Life Sciences Group, Institut Laue-Langevin, Grenoble Cedex 9, France; Macromolecular Structure Group, Keele University, Staffordshire, United Kingdom. Electronic address: tforsyth@ill.fr.
          Article
          S0076-6879(15)00637-0
          10.1016/bs.mie.2015.11.001
          26791978
          354dc6c0-b110-45f3-a61a-4f12e9048541
          © 2016 Elsevier Inc. All rights reserved.
          History

          Contrast variation,Deuteration,Dynamics,Isotope labelling,Neutron crystallography,Neutron reflection,Neutron scattering,SANS,Structural biology

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