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Quantitative analysis of catechins in Saraca asoca and correlation with antimicrobial activity

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      Abstract

      Herbal medicines are highly complex and have unknown mechanisms in diseases treatment. Saraca asoca (Roxb.), De. Wild has been recommended to treat gynecological disorders and used in several commercial polyherbal formulations. In present study, efforts have been made to explore antimicrobial activity and its co-relation with the distributions of catechins in the organs of S. asoca using targeted MS/MS. Eight extracts (cold and hot water) from four different organs of S. asoca and two drugs were prepared and antimicrobial activity was assessed by microbroth dilution assay. Quantitative and qualitative analysis of catechins in crude extracts was done by using targeted and auto-MS/MS and correlated with antimicrobial activity. (+)-Catechin and (+)-epicatechin and their biosynthesis related compound were found to be up-regulated in regenerated bark and leaves extracts. (−)-Epigallocatechin was found to be significantly higher in bark water extract as compared to others but showed low antimicrobial activity. Result showed down-regulation of (−)-epigallocatechin and up-regulation of (+)-catechin and (+)-epicatechin in the regenerated bark and leaves of S. asoca. It might be the contributing factor in the antimicrobial activity of regenerated bark and leaves of the plant. The concentration of (+)-epicatechin in processed drugs (Ashokarishta) from Baidyanath was found to be seven times higher than that of Dabur Pvt. Ltd., but no antimicrobial activity was observed, indicating the variations among the plant based drugs. This will be helpful in rational use of S. asoca parts. Furthermore, the analytical method developed is sensitive, repeatable and reliable; therefore, it is suitable for quality control of herbal drugs.

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      Most cited references 15

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        Liquid chromatography coupled with time-of-flight and ion trap mass spectrometry for qualitative analysis of herbal medicines

        With the expansion of herbal medicine (HM) market, the issue on how to apply up-to-date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing great attention. Due to its inherent characteristics of accurate mass measurements and multiple stages analysis, the integrated strategy of liquid chromatography (LC) coupled with time-of-flight mass spectrometry (TOF-MS) and ion trap mass spectrometry (IT-MS) is well-suited to be performed as qualitative analysis tool in this field. The purpose of this review is to provide an overview on the potential of this integrated strategy, including the review of general features of LC-IT-MS and LC-TOF-MS, the advantages of their combination, the common procedures for structure elucidation, the potential of LC-hybrid-IT-TOF/MS and also the summary and discussion of the applications of the integrated strategy for HM qualitative analysis (2006–2011). The advantages and future developments of LC coupled with IT and TOF-MS are highlighted.
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            Author and article information

            Affiliations
            [a ]National Research Institute of Basic Ayurvedic Sciences, (Central Council for Research in Ayurvedic Sciences), Nehru Garden, Kothrud, Pune 411038, India
            [b ]Centre for Biotechnology, Maharishi Dayanand University, Rohtak, Haryana 124001, India
            [c ]Department of Biochemistry, Maharishi Dayanand University, Rohtak, Haryana 124001, India
            Author notes
            [* ]Corresponding author at: Department of Biochemistry, Maharishi Dayanand University, Rohtak, Haryana 124001, India. Tel.: +91 12 6239 3070. rajeshdabur@ 123456yahoo.com
            Contributors
            Journal
            J Pharm Anal
            J Pharm Anal
            Journal of Pharmaceutical Analysis
            Xi'an Jiaotong University
            2095-1779
            2214-0883
            09 February 2013
            December 2013
            09 February 2013
            : 3
            : 6
            : 421-428
            5760998 S2095-1779(13)00011-7 10.1016/j.jpha.2013.01.007
            © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V.

            This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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