Detecting the onset of functional overreaching (FOR) or nonfunctional overreaching in endurance athletes is of prior importance to ensure reactive amendment of the scheduled training program. The objective of this study was to assess photoplethysmography (PPG) in overloaded athletes and test whether 1) it would be affected differently in functional overreached (FOR) or nonoverreached acutely fatigued (AF) athletes and 2) specific PPG characteristics would allow for timely distinction of FOR and AF.