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Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars
Karabi Datta ,
Jumin Tu ,
Norman Oliva ,
Isabelita Ona ,
Rethinasamy Velazhahan ,
Twng Wah Mew ,
Subbaratnam Muthukrishnan ,
Swapan K Datta
February 2001
February 2001
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Abstract
Genetic transformation has been attempted for management of rice sheath blight disease,
caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated
from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B,
MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system.
Inheritance was studied up to the T(2) generation by Southern blot analysis. Western
blot analysis of transgenic plants with polyclonal antibody revealed the presence
of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase
antibody. The transformants synthesized different levels of chitinase proteins constitutively
and progeny from the plants containing the chitinase gene showed different levels
of enhanced resistance when challenged with the sheath blight pathogen R. solani.