Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants

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Abstract

Background

Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Artabotrys burmanicus A.DC, Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders and Dasymaschalon sp. have been used for traditional medicine to treat cancer-like symptoms in some ethnic groups of Thailand and Laos.

Methods

We evaluated the anti-cancer activity of these Annonaceae plants against several human cancer cell lines. The apoptosis induction was detected by Annexin/propidium iodide (PI) staining. Phytochemical screening was tested by standard protocols and bioactive compounds were determined by HPLC.

Results

The crude extracts from leaves of U. longipes, Dasymaschalon sp., A. burmanicus, and M. modestum showed particular effects that were found to vary depending on the cancer cell line, suggesting that the effect was in a cell-type specific manner. Interestingly, the induction of apoptotic cell death was prominent by the leaves-derived crude extract of M. modestum. This crude was, therefore, subjected to cell cycle analysis by PI staining. Results showed that this crude extract arrested cell cycle and increased the percentage of cells in the SubG1 phase in some cancer cell lines. The phytochemical screening tests indicated that all crude extracts contained tannins and flavonoids. HPLC of flavonoids using standards identified rutin as an active compound in U. longipes and Dasymaschalon sp., whereas quercetin was found in U. longipes and M. modestum.

Conclusions

These crude extracts provide a new source for rutin and quercetin, which might be capable of inducing cancer cell apoptotic death in a cell-type specific manner. This suggests, by analyzing the major bioactive compounds, the potential use of these crudes for chemotherapy in the future.

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Most cited references 39

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Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009.

G Kroemer, L Galluzzi, P Vandenabeele … (2009)

Different types of cell death are often defined by morphological criteria, without a clear reference to precise biochemical mechanisms. The Nomenclature Committee on Cell Death (NCCD) proposes unified criteria for the definition of cell death and of its different morphologies, while formulating several caveats against the misuse of words and concepts that slow down progress in the area of cell death research. Authors, reviewers and editors of scientific periodicals are invited to abandon expressions like 'percentage apoptosis' and to replace them with more accurate descriptions of the biochemical and cellular parameters that are actually measured. Moreover, at the present stage, it should be accepted that caspase-independent mechanisms can cooperate with (or substitute for) caspases in the execution of lethal signaling pathways and that 'autophagic cell death' is a type of cell death occurring together with (but not necessarily by) autophagic vacuolization. This study details the 2009 recommendations of the NCCD on the use of cell death-related terminology including 'entosis', 'mitotic catastrophe', 'necrosis', 'necroptosis' and 'pyroptosis'.

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Life or death: neuroprotective and anticancer effects of quercetin.

Federico Dajas (2012)

Quercetin is a ubiquitous flavonoid that is present in numerous plants that are utilized in many different cultures for their nervous system and anticancer effects. To better understand the neuroprotective and antiproliferative activities of quercetin, we present a comprehensive review of the divergent actions that contribute to the ethnopharmacological profile of these plants. The pharmacological activities of quercetin that modulate antioxidation/oxidation/kinase-signaling pathways might be differentially elicited in neurons compared with malignant cells, ultimately promoting cell survival or death in a cell type- and metabolism-specific manner. Whereas the broad antioxidation and anti-inflammatory activities of quercetin are important for neuronal survival, the oxidative, kinase- and cell cycle-inhibitory, apoptosis-inducing effects of quercetin are essential for its anticancer effects. The diverse mechanistic interactions and activities of quercetin that modulate the phosphorylation state of molecules as well as gene expression would alter the interconnected and concerted intracellular signaling equilibrium, either inhibiting or strengthening survival signals. These mechanisms, which have been mainly observed in in vitro studies, cannot be easily translated into an explanation of the divergent simultaneous neuroprotective and anticancer effects observed in vivo. This is in part due to low bioavailability in plasma and in the brain, as well as the nature of the actual active molecules. Numerous studies have demonstrated the beneficial effects of chronic quercetin intake, which is ethnopharmacologically meaningful, as many plants that are chronically ingested by people contain quercetin. Although quercetin and quercetin-containing plants exhibit potential as therapeutic modalities in neuropathology and in cancer, the data collectively highlight the need to elucidate issues such as bioavailability as well as its correlation with effectiveness at biomarkers in vivo. There would be an increased potentential of these plants for chemoprevention and neuropathology prevention. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

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A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs.

Yitao Wang, Keli Xun, Xiuping Chen … (2009)

Natural products represent a rich reservoir of potential small chemical molecules exhibiting antiproliferation and anticancer properties. An example is berberine, a protoberberine alkaloid widely distributed in medical plants used in traditional Chinese prescriptions. Recent advances have shown that berberine exerts anticancer activities both in vitro and in vivo through different mechanisms. Berberine shows inhibitory effects on the proliferation and reproduction of certain tumorigenic microorganisms and viruses, such as Heliobacter pylori and hepatitis B virus. Transcriptional regulation of some oncogene and carcinogenesis-related gene expression and interaction with both DNA and RNA are also well documented. Besides, berberine is a broad spectrum enzyme inhibitor, which affects N-acetyltransferase, cyclooxygenase-2, and topoisomerase activities and gene/protein expression. These actions, together with the regulation of reactive oxygen species production, mitochondrial transmembrane potential, and nuclear factor-kappaB activation might underlie its antiproliferative and proapoptotic effects. More importantly, the suppression of tumor growth and metastasis, the beneficial application in combined medication, and the improvement of multidrug resistance both in vivo and in vitro clearly show its potential as an alternative medicine for tumor chemotherapy.

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Author and article information

Contributors

Kitti Pumiputavon: nokkumagi@gmail.com

Tanawat Chaowasku: cyathostemma@yahoo.com

Chalermpong Saenjum: chalermpong.saenjum@gmail.com

Maslin Osathanunkul: omaslin@gmail.com

Boonsong Wungsintaweekul: boonsong.wu@wu.ac.th

Kriangkrai Chawansuntati: heabjung@hotmail.com

Jiraprapa Wipasa: jwipasa@hotmail.com

Pathrapol Lithanatudom: pathrapol_li@hotmail.com

Journal

Journal ID (nlm-ta): BMC Complement Altern Med

Journal ID (iso-abbrev): BMC Complement Altern Med

Title: BMC Complementary and Alternative Medicine

Publisher: BioMed Central (London )

ISSN (Electronic): 1472-6882

Publication date (Electronic): 5 June 2017

Publication date PMC-release: 5 June 2017

Publication date Collection: 2017

Volume: 17

Electronic Location Identifier: 294

Affiliations

[1 ]ISNI 0000 0000 9039 7662, GRID grid.7132.7, Department of Biology, Faculty of Science, , Chiang Mai University, ; Chiang Mai, 50200 Thailand

[2 ]ISNI 0000 0000 9039 7662, GRID grid.7132.7, Department of Pharmaceutical Science, Faculty of Pharmacy, , Chiang Mai University, ; Chiang Mai, 50200 Thailand

[3 ]ISNI 0000 0001 0043 6347, GRID grid.412867.e, School of Pharmacy, , Walailak University, ; Nakhon Si Thammarat, 80160 Thailand

[4 ]ISNI 0000 0000 9039 7662, GRID grid.7132.7, Research Institute for Health Sciences, , Chiang Mai University, ; Chiang Mai, 50200 Thailand

Article

Publisher ID: 1811

DOI: 10.1186/s12906-017-1811-3

PMC ID: 5460496

PubMed ID: 28583139

SO-VID: 35a656f3-074f-4825-9856-465109dcdd23

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 23 March 2017

Date accepted : 26 May 2017

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100004396, Thailand Research Fund;

Award ID: MRG5680080

Award Recipient : Pathrapol Lithanatudom

Funded by: Chiang Mai University Research Group Grant

Funded by: The Research University Program of Chiang Mai University under Thailand’s Office of Higher Education Commission

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ScienceOpen disciplines: Complementary & Alternative medicine

Keywords: annonaceae,anti-cancer activity,cell cycle arrest,apoptosis,quercetin,rutin

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ScienceOpen disciplines: Complementary & Alternative medicine

Keywords: annonaceae, anti-cancer activity, cell cycle arrest, apoptosis, quercetin, rutin

Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants

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Abstract

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The International Journal of Person Centered Medicine

Most cited references 39

Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009.

Life or death: neuroprotective and anticancer effects of quercetin.

A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs.

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