Principles and techniques of digital holographic microscopy

Kim, Myung K.

doi:10.1117/6.0000006

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Principles and techniques of digital holographic microscopy

Author(s): Myung K. Kim

Publication date (Print): April 2010

Journal: Journal of Photonics for Energy

Publisher: SPIE-Intl Soc Optical Eng

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A new microscopic principle.

D. Gabor (1948)

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Tomographic phase microscopy.

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan … (2007)

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.