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      DNA Damage-Induced Phosphorylation of p53 Alleviates Inhibition by MDM2

      , , ,
      Cell
      Elsevier BV

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          Abstract

          DNA-damaging agents signal to p53 through as yet unidentified posttranscriptional mechanisms. Here we show that phosphorylation of human p53 at serine 15 occurs after DNA damage and that this leads to reduced interaction of p53 with its negative regulator, the oncoprotein MDM2, in vivo and in vitro. Furthermore, using purified DNA-dependent protein kinase (DNA-PK), we demonstrate that phosphorylation of p53 at serines 15 and 37 impairs the ability of MDM2 to inhibit p53-dependent transactivation. We present evidence that these effects are most likely due to a conformational change induced upon phosphorylation of p53. Our studies provide a plausible mechanism by which the induction of p53 can be modulated by DNA-PK (or other protein kinases with similar specificity) in response to DNA damage.

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          Author and article information

          Journal
          Cell
          Cell
          Elsevier BV
          00928674
          October 1997
          October 1997
          : 91
          : 3
          : 325-334
          Article
          10.1016/S0092-8674(00)80416-X
          9363941
          35fe80da-69dc-47c1-a6bc-bcd4e11ce167
          © 1997

          https://www.elsevier.com/tdm/userlicense/1.0/

          https://www.elsevier.com/open-access/userlicense/1.0/

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