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      Glycation of Human Lens Crystallins: Effect of Age and Aspirin Treatment

      ,

      Ophthalmic Research

      S. Karger AG

      Glycation, Crystallins, Aspirin, Lens, Acetylation

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          Abstract

          Human lenses of three different ages were used to study the effect of age and aspirin treatment on glycation of α-, β- and γ-crystallins. Soluble lens proteins were subjected to in vitro glycation with 5 m M [<sup>14</sup>C]glucose in the presence and absence of 10 m M aspirin. With crystallins from a 27-year-old lens α-crystallin was the most readily glycated protein. Glycation of all crystallins decreased substantially (37-77%) in 46- and 67-year-old lenses indicating an age-dependent decline in glycation sites. On the basis of a sensitive chemical assay for protein-bound glycogroups in lenses of 2-82 years of age this decline is apparently due to a 60% increase in in vivo glycation. Aspirin did not show any selectivity with regard to its ability to inhibit glycation of various crystallins. Irrespective of the age glycation of all crystallins was inhibited to a varying extent.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1993
          1993
          11 December 2009
          : 25
          : 6
          : 349-354
          Affiliations
          Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Ga., USA
          Article
          267336 Ophthalmic Res 1993;25:349–354
          10.1159/000267336
          8309673
          © 1993 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 6
          Categories
          Original Paper

          Vision sciences, Ophthalmology & Optometry, Pathology

          Glycation, Acetylation, Lens, Aspirin, Crystallins

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