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      Estimación de interferencias en la determinación de fructosamina en suero de felinos y caninos Translated title: Estimation of interferences in the determination of fructosamine of feline and canine serum

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          Abstract

          La concentración de fructosamina en suero es útil para monitorear el control de la glucemia en un período precedente.En muestras de sangre, la presencia de hemoglobina y bilirrubina pueden causar interferencias en técnicas analíticas diagnósticas. Por ello, el objetivo de este trabajo fue evaluar la posible interferencia al medir fructosamina sérica de gatos y perros clínicamente sanos. Se construyeron interferogramas, agregando concentraciones crecientes del posible interferente a pools de sueros de ambas especies. Se estudiaron concentraciones de hemoglobina desde 0.19 a 7.5 g/l en felinos, y de 0.16 a 6.10 g/l en caninos. Para bilirrubina, desde 6 a 600 mg/l, en ambas especies. Los resultados mostraron interferencia positiva y aditiva de hemoglobina en felinos (≥1.60 g/l) e interferencia negativa en caninos (≥1.20 g/l). En ambas especies se observaron interferencias positivas y aditivas para bilirrubina en el rango de concentraciones estudiadas (≥30 mg/l para felinos y ≥150 mg/l para caninos).

          Translated abstract

          The fructosamine serum concentration reflects the degree of glycemic control obtained during a preceding period. Usually, the presence of hemoglobin or bilirrubin in blood samples, could cause interferences in the laboratory analytical assays.Thus, we investigated the possible interferences on fructosamine determinations in serum from healthy cats and dogs. To testing and quantify the interference, we construct interferograms adding increased concentrations of hemoglobin or bilirrubin to serum pools of cats or dogs. We tested serum hemoglobin concentrations from 0.19 to 7.5 g/l in feline sera and 0.16 to 6.10 g/l in canine sera. The bilirrubin testing was done from 6 to 600 mg/l in both species sera. The results had shown a hemoglobin positive and additive interference in feline sera (≥1.60 g/l) and a negative interference in canine sera (≥1.20 g/l). In addition both species had shown positive and additive bilirrubin interferences (≥30 mg/l for feline sera and ≥150 mg/l for canine sera)

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          Most cited references 13

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          The ready isomerization of bilirubin IX- in aqueous solution.

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            Interference with clinical laboratory analyses.

            Interference by endogenous and exogenous substances with assays for clinical analytes is a common problem in laboratory medicine. For this review, we defined interference as "the effect of a substance present in the sample that alters the correct value of the result, usually expressed as concentration or activity, for an analyte." There are four major endogenous compounds that consistently interfere with laboratory results: hemoglobin, bilirubin, lipids, and paraproteins. The major exogenous sources of interference are drugs prescribed for the patient; and there are several excellent compendia of the effect of drugs on clinical laboratory tests. We recommend determining whether the interference is dependent or independent of the analyte for the assay. Further, we propose an approach to the identification and resolution of an interference problem for the clinical laboratory and make recommendations to manufacturers.
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              Graphical comparisons of interferences in clinical chemistry instrumentation.

              Instrument- and analyte-specific interferences can be produced by adding known concentrations of potentially interfering substances to serum from healthy volunteers. Analytical results are expressed as a percentage of the original (unaffected) result. Graphical displays of the transformed data document the conditions under which erroneous results would be probable for specimens containing the additive. If several analytical systems are available, one can make an informed choice of which to use for a particular analysis by comparing the appropriate graphical information. Comparisons of such displays of data from newer systems being considered for purchase may facilitate decisions regarding laboratory instrumentation.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                invet
                InVet
                InVet
                Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (Ciudad Autónoma de Buenos Aires, , Argentina )
                1668-3498
                December 2014
                : 16
                : 2
                : 63-68
                Affiliations
                Casilda Santa Fe orgnameUNR orgdiv1Facultad de Ciencias Veterinarias orgdiv2Cátedra de Farmacología y Terapéutica Argentina
                Rosario orgnameUNR orgdiv1Facultad de Ciencias Veterinarias orgdiv2Centro Binacional Argentina
                Article
                S1668-34982014000200002

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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                Figures: 0, Tables: 0, Equations: 0, References: 13, Pages: 6
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                Artículos de investigación

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