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      Radioimmunoassay for Non-Suppressible Insulin-Like Activity


      Hormone Research in Paediatrics

      S. Karger AG

      NSILA-S, Radioimmunoassay, Bioassay, Plasma levels, Man, Rat

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          By immunizing rabbits – tolerant against the bulk of normal human serum proteins – with highly purified non-suppressible insulin-like activity (NSILA-S), an anti-serum was obtained which made possible the development of a double-antibody radioimmunoassay. Its sensitivity is about 30 pg NSILA-S per tube or 150 pg NSILA-S/ml. The specificity exceeds that of the bioassay used for comparison which is based on the stimulation by NSILA-S of <sup>125</sup>IUDR incorporation into chicken fibroblasts in culture. The radioimmunoassay is sufficiently sensitive and specific to allow direct NSILA-S measurement in serum or plasma samples of humans and experimental animals. In human plasma samples NSILA-S levels, varying between less than 0.15 and 25 ng/ml, were found to have an average of about 4 ng/ml. In rats higher levels were observed with a mean of 7.7 ng/ml in 4-week-old animals, increasing to about 60 ng/ml in 6-month-old rats. In fasting rats the NSILA-S plasma level is reduced. In acid-treated samples of plasma considerably higher NSILA-S amounts are found.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          25 November 2008
          : 7
          : 4-5
          : 201-213
          Pharmaceutical Research Department, F. Hoffmann-La Roche & Co. Ltd., Basel
          178730 Horm Res 1976;7:201–213
          © 1976 S. Karger AG, Basel

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          Page count
          Pages: 13


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