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      Densitometric analysis of the autogenous demineralized dentin matrix on the dental socket wound healing process in humans Translated title: Análise densitométrica da matriz dentinária desmineralizada autógena na reparação alveolar de humanos

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          Abstract

          The aim of this study was to evaluate the effects of the autogenous demineralized dentin matrix (ADDM) on the third molar socket wound healing process in humans, using the guided bone regeneration technique and a polytetrafluoroethylene barrier (PTFE). Twenty-seven dental sockets were divided into three groups: dental socket (Control), dental socket with PTFE barrier (PTFE), and dental socket with ADDM slices associated to PTFE barrier (ADDM + PTFE). The dental sockets were submitted to radiographic bone densitometry analysis and statistical analysis on the 15th, 30th, 60th and 90th days using analysis of variance (ANOVA) and Tukey's test (p £ 0.05). The radiographic analysis of the ADDM + PTFE group showed greater homogeneity of bone radiopacity than the Control group and the PTFE group, during all the observation times. The dentin matrix gradually disappeared from the dental socket during the course of the repair process, suggesting its resorption during the bone remodeling process. It was concluded that the radiographic bone density of the dental sockets treated with ADDM was similar to that of the surrounding normal bone on the 90th day. The ADDM was biocompatible with the bone tissue of the surgical wounds of human dental sockets. The radiographic analysis revealed that the repair process was discreetly faster in the ADDM + PTFE group than in the Control and PTFE groups, although the difference was not statistically significant. In addition, the radiographic image of the ADDM + PTFE group suggested that its bone architecture was better than that of the Control and PFTE groups.

          Translated abstract

          O objetivo desta pesquisa foi avaliar a reparação óssea em alvéolos dentários após exodontia dos terceiros molares inferiores em humanos, com implantação de matriz dentinária desmineralizada autógena (MDDA) na cavidade e cobertura desta com barreira de politetrafluoretileno (PTFE). Foram selecionados 27 dentes, os quais foram divididos em três grupos: alvéolo dentário (Controle), alvéolo dentário com barreira de PTFE (PTFE) e alvéolo dentário com fatias de MDDA associada à barreira de PTFE (MDDA + PTFE). O alvéolo dentário foi submetido à análise de densitometria radiográfica e à análise estatística no 15º, 30º, 60º e 90º dias, utilizando-se a análise de variância (ANOVA) e teste de Tukey (p < 0,05). A análise radiográfica do grupo MDDA + PTFE mostrou maior homogeneidade na radiopacidade do trabeculado ósseo do que no grupo controle e grupo PTFE, durante todos os períodos de observação. A matriz dentinária foi desaparecendo do alvéolo dentário durante a evolução do processo de reparo, sugerindo sua reabsorção durante o processo de remodelação óssea. Concluiu-se que a densidade óssea radiográfica do alvéolo dentário tratado com MDDA foi similar à do osso normal circunjacente no 90º dia. A MDDA foi biocompatível com o tecido ósseo, quando implantada nos alvéolos dentários cruentos de humanos. Na análise radiográfica, pode-se verificar que o processo de reparo foi discretamente mais rápido no grupo MDDA + PTFE do que no grupo Controle e no grupo PTFE, porém não houve diferença estatística significante. Além disso, a imagem radiográfica sugeriu que a arquitetura óssea do grupo MDDA + PTFE foi melhor do que a arquitetura óssea do grupo controle e grupo PTFE.

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          Most cited references13

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          Bovine tooth-derived bone morphogenetic protein.

          Eight groups of dental tissues were mechanically dissected from the mandibles of one-year-old steers; they were then defatted and decalcified in HCl. The noncollagenous proteins were extracted with various solvents from collections of tissue and bio-assayed for osteo-inductive activity. Collectively, the hard tissue (dentin, enamel, and cementum) noncollagenous proteins were fractionated by molecular sieve chromatography, hydroxyapatite affinity chromatography, and ion exchange chromatography. Osteo-inductive activity of each protein fraction was determined by implantation in the quadriceps muscle pouch of mice. The quantity of bone was measured by computerized image analysis. From 71% to 83% of 41 implants of dental hard tissues induced bone formation. The quantity of bone was greater from unerupted than from erupted teeth. Dental soft tissues that had no osteo-inductive activity were rich in a 14-kDa protein, presumably matrix gamma-carboxyglutamic acid-rich proteins. Proteins with Mr of from 15 to 28 kDa were associated with osteo-inductive activity. Components with Mr greater than 28 kDa had no activity. These observations suggest that bovine teeth have a selection of osteo-inductive proteins that is comparable in range of MW to bovine bone morphogenetic protein.
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            Induction of odontoblast-like cell differentiation in dog dental pulps after in vivo implantation of dentine matrix components.

            The effects of dentine extracellular matrix components on dental mesenchymal cells were studied by light and transmission electron microscopy after their implantation at central sites of mechanically exposed pulps in dog molar teeth. The implants were Millipore filters that had been soaked with solutions containing 30 or 300 micrograms/ml of an EDTA-soluble fraction of rabbit incisor dentine. Control filters were soaked with dog albumin or phosphate buffered saline. Columnar, polarized cells were consistently seen after 8 days in close proximity to the filters coated with both concentrations of dentine matrix components. Characteristic features of these polarized cells included widened cisternae of the rough endoplasmic reticulum, a rich microfilamentous network in the long cytoplasmic extensions invading the filter pores and numerous cytoplasmic bodies. These cells also showed evidence of functional as well as cytological differentiation. Polarized processing of secretory granules could be observed after 8 days' implantation, and also the presence of matrix vesicles and deposition of a fine, collagenous matrix into the filters apically to the distal end of the cytoplasmic processes. After 24 days' implantation, secretion of a tubular matrix could be consistently seen in association with the odontoblast-like cells. No changes in cell organization or matrix synthesis were seen after implantation of control filters. These studies demonstrate that bioactive components present in the EDTA-soluble dentine matrix fraction are able to directly induce cell polarization and apical secretion of tubular matrix when implanted in contact with dental pulp cells at sites remote from the odontoblast layer.
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              Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction

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                Author and article information

                Journal
                bor
                Brazilian Oral Research
                Braz. oral res.
                Sociedade Brasileira de Pesquisa Odontológica - SBPqO (São Paulo, SP, Brazil )
                1806-8324
                1807-3107
                December 2006
                : 20
                : 4
                : 324-330
                Affiliations
                [04] orgnameSão Paulo State University orgdiv1School of Dentistry of São José dos Campos orgdiv2Department of Surgery, Periodontology and Radiology
                [02] orgnameSão Paulo State University orgdiv1School of Dentistry of São José dos Campos orgdiv2Bioscience Center for Special Health Care Needs (CEBAPE)
                [05] orgnameSão Paulo State University orgdiv1School of Dentistry of São José dos Campos orgdiv2Bioscience Center for Special Health Care Needs (CEBAPE)
                [01] orgnameSpecial Health Care Needs Association (ASPE)
                [03] orgnameSão Paulo State University orgdiv1School of Dentistry of São José dos Campos
                Article
                S1806-83242006000400008 S1806-8324(06)02000408
                10.1590/S1806-83242006000400008
                372d3f6e-cfed-482e-b078-06e5141d9d4b

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 15 February 2006
                : 20 July 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 13, Pages: 7
                Product

                SciELO Brazil

                Categories
                Radiology

                Tissue engineering,Dentin,Bone regeneration,Engenharia tissular,Dentina,Regeneração óssea

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