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      Three-Dimensional Ultrastructure of Human Glomerular Basement Membrane by a Quick-Freezing and Deep-Etching Method

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          Abstract

          The three-dimensional ultrastructure of glomerular basement membrane (GBM) in surgically resected human kidneys was observed by the quick-freezing and deep-etching method. In three layers of human GBM, the middle layer exhibited polygonal meshwork structures, which were composed of fine fibrils. In addition, the outer and inner layers were formed by lines of perpendicular fibrils, which connected the epithelial or endothelial cell surfaces with the meshwork structures of the middle layer. These ultrastructures might correspond to the three layers, such as lamina densa and laminae rara externa and interna, which were also observed on conventional ultrathin sections. It is concluded that the human GBM has the same meshwork structures and perpendicular fibrils as those previously observed in animals and that these structures may act functionally as size and/or charge barriers.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1995
          1995
          18 December 2008
          : 70
          : 1
          : 72-76
          Affiliations
          aDepartment of Internal Medicine, Kitasato University School of Medicine, Kitasato, and bDepartment of Anatomy, Yamanashi Medical University, Yamanashi, Japan
          Article
          188547 Nephron 1995;70:72–76
          10.1159/000188547
          7617120
          © 1995 S. Karger AG, Basel

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          Page count
          Pages: 5
          Categories
          Original Paper

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