Blog
About

114
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics.

      Journal of Lipid Research

      Animals, Brain Chemistry, Caenorhabditis elegans, Chromatography, Thin Layer, Escherichia coli, chemistry, Humans, Indicators and Reagents, Lipids, blood, isolation & purification, Mass Spectrometry, Methyl Ethers, Mice, Phospholipids, classification

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery of lipid species from analyzed cells, fluids, or tissues and is usually achieved by two-phase extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) extraction allows faster and cleaner lipid recovery and is well suited for automated shotgun profiling. Because of MTBE's low density, lipid-containing organic phase forms the upper layer during phase separation, which simplifies its collection and minimizes dripping losses. Nonextractable matrix forms a dense pellet at the bottom of the extraction tube and is easily removed by centrifugation. Rigorous testing demonstrated that the MTBE protocol delivers similar or better recoveries of species of most all major lipid classes compared with the "gold-standard" Folch or Bligh and Dyer recipes.

          Related collections

          Author and article information

          Journal
          18281723
          2311442
          10.1194/jlr.D700041-JLR200

          Comments

          Comment on this article