7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Physical and genetic characterization of the genome of Lactobacillus lactis bacteriophage LL-H.

      Journal of Biology
      Bacteriolysis, Bacteriophages, genetics, metabolism, DNA Restriction Enzymes, DNA, Viral, Escherichia coli, Genes, Viral, Lactobacillus, Promoter Regions, Genetic, Viral Proteins, biosynthesis, Viral Structural Proteins

      Read this article at

      ScienceOpenPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Bacteriophage LL-H is a virulent phage of Lactobacillus lactis LL23. A restriction map of the phage genome was constructed with various restriction endonucleases. This chromosome has a 34-kilobase size and seems to be circularly permuted. We used a bank of LL-H restriction fragments to study the expression of five of the seven main phage particle proteins. Immunoblotting experiments permitted the mapping on the chromosome of several genes coding for phage particle proteins. We also show that the gene of the main capsid protein is expressed from its own promoter in an Escherichia coli strain.

          Related collections

          Author and article information

          Comments

          Comment on this article