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      Meshwork Structures in Bovine Glomerular and Tubular Basement Membrane as Revealed by Ultra-High Resolution Scanning Electron Microscopy

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          Abstract

          We examined the ultrastructure of the bovine glomerular basement membrane (GBM) and tubular basement membrane (TBM) using ultra-high-resolution scanning electron microscopy after conductive staining without metal coating. Purified basement membranes (BMs) were obtained by sonication and acellular BMs by detergent treatments. Purified GBMs (PGBMs) and acellular GBMs (AGBMs) showed similar meshwork structures composed of regular round or oval pores and branching strands. Pore diameters were 10.2 ± 2.4 nm (mean ± SD) in PGBMs and 9.8 ± 3.1 nm in AGBMs. Purified TBMs (PTBMs) and acellular TBMs (ATBMs) exhibited heterogeneous meshwork structures in which compact meshes in the cristae were combined with coarse meshes in the invaginations on the epithelial surfaces. Pore diameters were 12.6 ± 5.2 nm in PTBMs and 11.4 ± 4.0 nm in ATBMs which were significantly larger than those in GBMs. The width of the strands ranged from 3 to 15 nm in all BMs. A 4 M guanidine hydrochloride extraction of the acellular BMs revealed large polygonal networks in the invaginations of the TBM and twisted strands which were considered to be type IV collagen fibrils. Ultra-high-resolution scanning electron microscopy and conductive staining were useful for the study of three-dimensional architectures of BMs and revealed heterogeneous meshwork structures in the GBM and TBM which were probably caused by a different ratio of the major components.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1994
          1994
          16 December 2008
          : 66
          : 2
          : 189-199
          Affiliations
          Third Department of Internal Medicine, Okayama University Medical School, Okayama, Japan
          Article
          187800 Nephron 1994;66:189–199
          10.1159/000187800
          8139740
          © 1994 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Original Paper

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