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      Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat

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          Summary

          Despite a long history, the production of useful alien introgression lines in wheat remains difficult mainly due to linkage drag and incomplete genetic compensation. In addition, little is known about the molecular mechanisms underlying the impact of foreign chromatin on plant phenotype. Here, a comparison of the transcriptomes of barley, wheat and a wheat–barley 7 HL addition line allowed the transcriptional impact both on 7 HL genes of a non‐native genetic background and on the wheat gene complement as a result of the presence of 7 HL to be assessed. Some 42% (389/923) of the 7 HL genes assayed were differentially transcribed, which was the case for only 3% (960/35 301) of the wheat gene complement. The absence of any transcript in the addition line of a suite of chromosome 7A genes implied the presence of a 36 Mbp deletion at the distal end of the 7 AL arm; this deletion was found to be in common across the full set of Chinese Spring/Betzes barley addition lines. The remaining differentially transcribed wheat genes were distributed across the whole genome. The up‐regulated barley genes were mostly located in the proximal part of the 7 HL arm, while the down‐regulated ones were concentrated in the distal part; as a result, genes encoding basal cellular functions tended to be transcribed, while those encoding specific functions were suppressed. An insight has been gained into gene transcription in an alien introgression line, thereby providing a basis for understanding the interactions between wheat and exotic genes in introgression materials.

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          Most cited references 56

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          Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat.

          The foundation of western civilization owes much to the high fertility of bread wheat, which results from the stability of its polyploid genome. Despite possessing multiple sets of related chromosomes, hexaploid (bread) and tetraploid (pasta) wheat both behave as diploids at meiosis. Correct pairing of homologous chromosomes is controlled by the Ph1 locus. In wheat hybrids, Ph1 prevents pairing between related chromosomes. Lack of Ph1 activity in diploid relatives of wheat suggests that Ph1 arose on polyploidization. Absence of phenotypic variation, apart from dosage effects, and the failure of ethylmethane sulphonate treatment to yield mutants, indicates that Ph1 has a complex structure. Here we have localized Ph1 to a 2.5-megabase interstitial region of wheat chromosome 5B containing a structure consisting of a segment of subtelomeric heterochromatin that inserted into a cluster of cdc2-related genes after polyploidization. The correlation of the presence of this structure with Ph1 activity in related species, and the involvement of heterochromatin with Ph1 (ref. 6) and cdc2 genes with meiosis, makes the structure a good candidate for the Ph1 locus.
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            Genetic Control of the Cytologically Diploid Behaviour of Hexaploid Wheat

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              changepoint: AnRPackage for Changepoint Analysis

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                Author and article information

                Contributors
                dolezel@ueb.cas.cz
                Journal
                Plant Biotechnol J
                Plant Biotechnol. J
                10.1111/(ISSN)1467-7652
                PBI
                Plant Biotechnology Journal
                John Wiley and Sons Inc. (Hoboken )
                1467-7644
                1467-7652
                13 April 2018
                October 2018
                : 16
                : 10 ( doiID: 10.1111/pbi.2018.16.issue-10 )
                : 1767-1777
                Affiliations
                [ 1 ] Institute of Experimental Botany Centre of the Region Haná for Biotechnological and Agricultural Research Olomouc Czech Republic
                [ 2 ] Agriculture Research Victoria Department of Economic Development Jobs Transport and Resources AgriBio Bundoora VIC 3083 Australia
                [ 3 ] Institute of Plant Sciences Paris Saclay IPS2 CNRS INRA Université Paris‐Sud Université Evry Université Paris‐Saclay Orsay France
                [ 4 ] Institute of Plant Sciences Paris‐Saclay IPS2 Paris Diderot Sorbonne Paris‐Cité Orsay France
                [ 5 ] IRHS Université d'Angers INRA AGROCAMPUS‐Ouest SFR4207 QUASAV Université Bretagne Loire Beaucouzé France
                [ 6 ] UMR MIA‐Paris AgroParisTech INRA Université Paris‐Saclay Paris France
                [ 7 ] Department of Plant Life Science Faculty of Agriculture Ryukoku University Shiga Japan
                [ 8 ] Murdoch University Perth WA Australia
                Author notes
                [* ] Correspondence (Tel +420 585 238 703; fax +420 585 238 704; email dolezel@ 123456ueb.cas.cz )
                Article
                PBI12913
                10.1111/pbi.12913
                6131412
                29510004
                © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

                This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                Page count
                Figures: 6, Tables: 1, Pages: 11, Words: 8675
                Product
                Funding
                Funded by: National Grid Infrastructure MetaCentrum
                Award ID: LM2010005
                Funded by: Ministry of Education, Youth and Sports of the Czech Republic
                Award ID: LO1204
                Funded by: Czech Science Foundation
                Award ID: P501/12/G090
                Categories
                Research Article
                Research Articles
                Custom metadata
                2.0
                pbi12913
                October 2018
                Converter:WILEY_ML3GV2_TO_NLMPMC version:version=5.4.7.1 mode:remove_FC converted:11.09.2018

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