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Development of high performance liquid chromatography method for buspirone in rabbit serum: Application to pharmacokinetic study
Ramesh Gannu ,
Shravan Kumar Yamsani ,
Chinna Reddy Palem ,
Vamshi Vishnu Yamsani ,
Harshini Kotagiri ,
Madhusudan Rao Yamsani
August 2009
August 2009
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Abstract
A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification
of buspirone (BUSP) in rabbit serum was developed and validated. BUSP and internal
standard (IS), diltiazem hydrochloride were extracted into dichloromethane and separated
using an isocratic mobile phase, on a Kromasil C(8) column. The eluent was monitored
by UV detector at 235 nm and at a flow rate of 1.0 mL min(-1). The linearity range
of proposed method was 1-3000 ng mL(-1). The intra-day and inter-day coefficient of
variation and percent error values of the assay method were less than 15% and mean
recovery was more than 97 and 96% for BUSP and IS, respectively. The method was found
to be precise, accurate, and specific during the study. The method was successfully
applied for pharmacokinetic study of buspirone after application of reservoir based
transdermal therapeutic system of BUSP to rabbits.