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      Involvement of flocculin in negative potential-applied ITO electrode adhesion of yeast cells

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          Abstract

          The purpose of this study was to develop novel methods for attachment and cultivation of specifically positioned single yeast cells on a microelectrode surface with the application of a weak electrical potential. Saccharomyces cerevisiae diploid strains attached to an indium tin oxide/glass (ITO) electrode to which a negative potential between −0.2 and −0.4 V vs. Ag/AgCl was applied, while they did not adhere to a gallium-doped zinc oxide/glass electrode surface. The yeast cells attached to the negative potential-applied ITO electrodes showed normal cell proliferation. We found that the flocculin FLO10 gene-disrupted diploid BY4743 mutant strain ( flo10Δ / flo10Δ) almost completely lost the ability to adhere to the negative potential-applied ITO electrode. Our results indicate that the mechanisms of diploid BY4743 S. cerevisiae adhesion involve interaction between the negative potential-applied ITO electrode and the Flo10 protein on the cell wall surface. A combination of micropatterning techniques of living single yeast cell on the ITO electrode and omics technologies holds potential of novel, highly parallelized, microchip-based single-cell analysis that will contribute to new screening concepts and applications.

          Abstract

          Yeast Saccharomyces cerevisiae were selectively attached on the negative potential-applied indium tin oxide/glass electrode. Mechanisms of the yeast cell attachment involve Flocculin Flo10 proteins.

          Abstract

          Graphical Abstract Figure.

          Yeast Saccharomyces cerevisiae were selectively attached on the negative potential-applied indium tin oxide/glass electrode. Mechanisms of the yeast cell attachment involve Flocculin Flo10 proteins.

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          Soft lithography in biology and biochemistry.

          G. M. Whitesides, E Ostuni, S. Takayama … (2001)
          Soft lithography, a set of techniques for microfabrication, is based on printing and molding using elastomeric stamps with the patterns of interest in basrelief. As a technique for fabricating microstructures for biological applications, soft lithography overcomes many of the shortcomings of photolithography. In particular, soft lithography offers the ability to control the molecular structure of surfaces and to pattern the complex molecules relevant to biology, to fabricate channel structures appropriate for microfluidics, and to pattern and manipulate cells. For the relatively large feature sizes used in biology (> or = 50 microns), production of prototype patterns and structures is convenient, inexpensive, and rapid. Self-assembled monolayers of alkanethiolates on gold are particularly easy to pattern by soft lithography, and they provide exquisite control over surface biochemistry.
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            Life with 6000 genes.

            A Goffeau, B G Barrell, H Bussey … (1996)
            The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 potential protein-encoding genes, approximately 140 genes specifying ribosomal RNA, 40 genes for small nuclear RNA molecules, and 275 transfer RNA genes. In addition, the complete sequence provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history. The genome shows a considerable amount of apparent genetic redundancy, and one of the major problems to be tackled during the next stage of the yeast genome project is to elucidate the biological functions of all of these genes.
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              Inkjet printing of viable mammalian cells.

              Tao Xu, Joyce Jin, Cassie Gregory … (2005)
              The purpose of this study was to explore the use of a commercial thermal printer to deposit Chinese Hamster Ovary (CHO) and embryonic motoneuron cells into pre-defined patterns. These experiments were undertaken to verify the biocompatibility of thermal inkjet printing of mammalian cells and the ability to assemble them into viable constructs. Using a modified Hewlett Packard (HP) 550C computer printer and an HP 51626a ink cartridge, CHO cells and rat embryonic motoneurons were suspended separately in a concentrated phosphate buffered saline solution (3 x). The cells were subsequently printed as a kind of "ink" onto several "bio-papers" made from soy agar and collagen gel. The appearance of the CHO cells and motoneurons on the bio-papers indicated an healthy cell morphology. Furthermore, the analyses of the CHO cell viability showed that less than 8% of the cells were lysed during printing. These data indicate that mammalian cells can be effectively delivered by a modified thermal inkjet printer onto biological substrates and that they retain their ability to function. The computer-aided inkjet printing of viable mammalian cells holds potential for creating living tissue analogs, and may eventually lead to the construction of engineered human organs. Copyright 2004 Elsevier Ltd.
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                Author and article information

                Journal
                Journal ID (nlm-ta): FEMS Yeast Res
                Journal ID (iso-abbrev): FEMS Yeast Res
                Journal ID (hwp): femsyr
                Journal ID (publisher-id): femsyr
                Title: FEMS Yeast Research
                Publisher: Oxford University Press
                ISSN (Print): 1567-1356
                ISSN (Electronic): 1567-1364
                Publication date (Electronic): 17 July 2015
                Publication date (Print): September 2015
                Publication date PMC-release: 17 July 2015
                Volume: 15
                Issue: 6
                Electronic Location Identifier: fov064
                Affiliations
                [1 ]Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
                [2 ]Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
                [3 ]Department of Marine Science, Marine Works Japan Ltd., 3-54-1 Oppamahigashi, Yokosuka 237-0063, Japan
                [4 ]Marine Technology and Engineering Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
                [5 ]Department of Foods and Human Nutrition, Notre Dame Seishin University, 2-16-9 Ifuku-cho, Kita-ku, Okayama 700-8516, Japan
                [6 ]Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan
                [7 ]Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan
                Author notes
                [* ] Corresponding author: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. Tel: +81-46-867-9691; Fax: +81-46-867-9525; E-mail: skoyama@ 123456jamstec.go.jp
                Article
                DOI: 10.1093/femsyr/fov064
                PMC ID: 4629795
                PubMed ID: 26187908
                SO-VID: 38490972-ebaf-4311-860a-acaade8d6436
                Copyright © © FEMS 2015.
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date accepted : 10 July 2015
                Date revision received : 08 May 2015
                Page count
                Pages: 12
                Categories
                Subject: Research Article
                Custom metadata
                cover-date September 2015

                ScienceOpen disciplines: Molecular biology
                Keywords: saccharomyces cerevisiae,flo10,electrical attachment,potential-controlled electrode,indium tin oxide,single-cell cultivation
                Data availability:
                ScienceOpen disciplines: Molecular biology
                Keywords: saccharomyces cerevisiae, flo10, electrical attachment, potential-controlled electrode, indium tin oxide, single-cell cultivation

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