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      Sodium-Potassium Pump Sites, as Assessed by [ 3H]-Ouabain Binding, in Aorta and Caudal Artery of Normotensive and Spontaneously Hypertensive Rats

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          Abstract

          The results of the present work provide support for the utility of studying [<sup>3</sup>H]- ouabain binding to in vitro segments of vascular smooth muscle as an indication of the number of Na-K pump sites in blood vessels of rats. Specific binding of [<sup>3</sup>H]-ouabain to strips of rat abdominal aorta and segments of rat caudal artery was saturable and reversible. Specific [<sup>3</sup>H]-ouabain binding was displaced by several cardiac glycosides but not by steroid hormones or sodium vanadate. The dissociation constant (K<sub>D</sub>) of [<sup>3</sup>H]-ouabain binding, as determined by equilibrium and kinetic studies, was in the range of 50–150 n M in these vessels. Scatchard analysis indicated that the maximum number of [<sup>3</sup>H]-ouabain-binding sites (B<sub>max</sub>) was 20 and 46 fmol/mg wet weight in the aorta strips and caudal artery, respectively. A comparison of [<sup>3</sup>H]-ouabain binding in caudal arteries of spontaneously hypertensive rats and of age-matched, genetic controls indicated that neither the affinity for binding nor the maximum number of binding sites was different. This suggests that this genetic form of hypertension is not associated with an alteration in the number of vascular Na-K pump sites.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1984
          1984
          23 September 2008
          : 21
          : 5
          : 211-222
          Affiliations
          Department of Pharmacology and Toxicology, West Virginia University Medical Center, Morgantown, W. Va., USA
          Article
          158514 Blood Vessels 1984;21:211–222
          10.1159/000158514
          © 1984 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 12
          Categories
          Research Paper

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