2
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Coronavirus Genome Structure and Replication

      chapter-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In addition to the SARS coronavirus (treated separately elsewhere in this volume), the complete genome sequences of six species in the coronavirus genus of the coronavirus family [avian infectious bronchitis virus-Beaudette strain (IBV-Beaudette), bovine coronavirus-ENT strain (BCoV-ENT), human coronavirus-229E strain (HCoV-229E), murine hepatitis virus-A59 strain (MHV-A59), porcine transmissible gastroenteritis-Purdue 115 strain (TGEV-Purdue 115), and porcine epidemic diarrhea virus-CV777 strain (PEDV-CV777)] have now been reported. Their lengths range from 27,317 nt for HCoV-229E to 31,357 nt for the murine hepatitis virus-A59, establishing the coronavirus genome as the largest known among RNA viruses. The basic organization of the coronavirus genome is shared with other members of the Nidovirus order (the torovirus genus, also in the family Coronaviridae, and members of the family Arteriviridae) in that the nonstructural proteins involved in proteolytic processing, genome replication, and subgenomic mRNA synthesis (transcription) (an estimated 14–16 end products for coronaviruses) are encoded within the 5′-proximal two-thirds of the genome on gene 1 and the (mostly) structural proteins are encoded within the 3′-proximal one-third of the genome (8–9 genes for coronaviruses). Genes for the major structural proteins in all coronaviruses occur in the 5′ to 3′ order as S, E, M, and N. The precise strategy used by coronaviruses for genome replication is not yet known, but many features have been established. This chapter focuses on some of the known features and presents some current questions regarding genome replication strategy, the cis-acting elements necessary for genome replication [as inferred from defective interfering (DI) RNA molecules], the minimum sequence requirements for autonomous replication of an RNA replicon, and the importance of gene order in genome replication.

          Related collections

          Most cited references118

          • Record: found
          • Abstract: not found
          • Article: not found

          Nidovirales: a new order comprising Coronaviridae and Arteriviridae.

          D Cavanagh (1997)
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            The molecular biology of arteriviruses.

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Upstream open reading frames as regulators of mRNA translation.

                Bookmark

                Author and article information

                Contributors
                L.Enjuanes@cnb.uam.es
                dbrian@utk.edu
                Journal
                978-3-540-26765-2
                10.1007/b138038
                Coronavirus Replication and Reverse Genetics
                Coronavirus Replication and Reverse Genetics
                978-3-540-21494-6
                978-3-540-26765-2
                2005
                : 287
                : 1-30
                Affiliations
                GRID grid.428469.5, ISNI 0000000417941018, Department of Molecular and Cell Biology, , Centro Nacional de Biotecnología, ; Campus Universidad Autónoma, Cantoblanco, 38049 Madrid, Spain
                [11 ]GRID grid.411461.7, ISNI 0000000123151184, Departments of Microbiology and Pathobiology, , University of Tennessee, College of Veterinary Medicine, ; Knoxville, TN 37996-0845 USA
                [12 ]GRID grid.10698.36, ISNI 0000000122483208, Department of Microbiology and Immunology, School of Medicine, , University of North Carolina, ; Chapel Hill, NV 27599-7400 USA
                [13 ]GRID grid.10698.36, ISNI 0000000122483208, Department of Epidemiology, Program of Infectious Diseases, School of Public Health, , University of North Carolina, ; Chapel Hill, NC 27599-7400 USA
                Article
                1
                10.1007/3-540-26765-4_1
                7120446
                15609507
                386c3aa8-e2f6-4c35-86e2-089d76872716
                © Springer-Verlag 2005

                This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

                History
                Categories
                Article
                Custom metadata
                © Springer-Verlag Berlin Heidelberg 2005

                infectious bronchitis virus,genome replication,mouse hepatitis virus,murine coronavirus,slippery sequence

                Comments

                Comment on this article