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      Vpr loosens chromatid ties

      The Journal of Cell Biology

      The Rockefeller University Press

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          Abstract

          Compared to cells lacking Vpr (left), the cohesin Rad21 (red) is prematurely lost from centromeres (arrowheads) in the presence of Vpr (right). An HIV-1 protein promotes chromosome missegregation by epigenetically modifying centromeric chromatin, Shimura et al. report. Lymphocytes infected with HIV-1 display premature chromatid separation (PCS), in which sister chromatids come apart too early in mitosis, increasing the possibility of missegregation into the wrong daughter cell. This genomic instability may explain the increased incidences of certain cancers in HIV-1 patients, though how HIV-1 induces PCS is unknown. Shimura et al. found that viruses lacking the small accessory gene vpr didn't disrupt chromatid cohesion, whereas expression of Vpr alone was sufficient to induce PCS. Cohesin proteins, which link sister chromatids together until anaphase, were prematurely lost from the centromeres of mitotic chromosomes, probably because hSgo1, a protein that protects cohesins from dissociation, was displaced in the presence of Vpr. hSgo1 is recruited to centromeres by heterochromatin proteins of the HP1 family. Shimura et al. saw that HP1-α and HP1-γ were displaced from centromeric chromatin by Vpr and that depleting HP1 proteins by RNAi caused similar levels of PCS. Vpr localized to chromosomes—especially at centromeres—and displaced HP1-α and -γ by recruiting the histone acetyltransferase p300 to modify the surrounding chromatin. Depleting or inhibiting p300 prevented Vpr from displacing HP1 proteins and inducing PCS. Blocking the interaction between Vpr and p300 may therefore reduce genomic instability in long-term HIV-1 patients. Senior author Mari Shimura now wants to investigate how the Vpr protein localizes to host cell chromosomes.

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          Author and article information

          Journal
          J Cell Biol
          jcb
          The Journal of Cell Biology
          The Rockefeller University Press
          0021-9525
          1540-8140
          5 September 2011
          : 194
          : 5
          : 658
          Author notes
          Article
          1945iti1
          10.1083/jcb.1945iti1
          3171118
          © 2011 The Rockefeller University Press
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          Cell biology

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