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      Movilidad individual de los espermatozoides epididimarios de toros postmortem obtenidos mediante lavado retrógrado Translated title: Individual motility of epidydimal spermatozoa, obtained by retrogadous flushing from post-mortem zebu bull testicles


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          El objetivo de este estudio fue evaluar la movilidad individual de los espermatozoides presentes en la cola del epidídimo, obtenida del matadero mediante lavado retrógrado de testículos de toros cebú postmortem. Los testículos fueron recolectados al azar (n=90) y transportados bajo dos diferentes temperaturas, siguiendo dos diferentes protocolos: Protocolo 1 (T: 35ºC) y Protocolo 2 (T: 25ºC), durante el período Abril-Julio 2003. El material experimental fue transportado según el protocolo elegido al azar, desde el sitio de recolección hasta el laboratorio en un lapso comprendido entre 30 y 60 minutos. Se realizó histopatología para seleccionar los testículos que tuvieran 70% o más de tejido funcional para cada protocolo (Protocolo 1: n=13; y Protocolo 2: n=20). Una vez realizada la selección, se evaluó la movilidad individual de los espermatozoides recolectados de las colas epididimarias en los testículos seleccionados. Esta recolección se realizó por lavado retrógrado con Tris-yema-glicerol, a través del conducto deferente. Para cada Protocolo se hicieron dos lecturas de la movilidad individual espermática (MI1 y MI2), con intervalo de 1 hora entre cada lectura.  Los datos se analizaron mediante estadística descriptiva y se realizó una prueba de t para comparar dos muestras independientes. Los resultados obtenidos indican que no hubo diferencia significativa (P<0,05) entre los dos Protocolos en el comportamiento de la movilidad individual. Esto nos indica que la temperatura de transporte no afecto la movilidad espermática epididimaria y que es posible recolectar espermatozoides vivos de la cola del epidídimo de toros postmortem.

          Translated abstract

          The objective of this study was to evaluate the individual motility of the spermatozoa of the epididymal tail, obtained by retrogradous flushing, from testicles of post mortem bulls collected from a slaughterhouse. The testicles (n=90) were randomly collected and transported under two different temperatures, following two different protocols: Protocol 1 (35ºC) and Protocol 2 (25ºC), during the April-July 2003 period. The testicles were transported from the slaughterhouse to the Laboratory, in a range of time between 30 and 60 minutes. A histopathological study was conducted to all testicles to select those with 70% or more of functional tissue. So, the final number of testicles for the study was reduced: for Protocol 1 to 13 testicles and for Protocol 2 to 20 testicles. The retrogradous flushing was done with Tris-egg yolk-glycerol medium, through the deferent duct. Two readings for motility were done for each protocol (M1 1 and MI 2) one hour apart. The data was analyzed by descriptive statistic and t test to compare two independents samples. There was no significant difference (P<0.05) between protocols on individual motility. This shows that, in this case, two different temperatures did not affect the individual motility of the spermatozoa of the epidydimal tail, and it is possible to obtain live spermatozoa from testicles from post mortem bulls.

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          Most cited references34

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          A critical review of the physiological importance and analysis of sperm movement in mammals.

          S Mortimer (2015)
          The identification of human sperm hyperactivated motility has potential importance in sperm function tests, as well as in quality control assays and in reproductive toxicology investigations. However, relatively little is known about this phenomenon and the variety of definitions used for hyperactivation has led to a great deal of confusion as to its occurrence and physiological relevance. This presentation is a critical review of a number of aspects of hyperactivated motility, including its identification and potential role(s) in mammalian fertilization. The initial sections of the review consider the mechanisms involved in the development and maintenance of mammalian sperm motility, and the structural and functional changes in spermatozoa which occur during transport through the female reproductive tract. The methods available for the quantification of aspects of sperm movement are also discussed, with an historical overview of sperm movement analysis.
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            A critical review of the physiological importance and analysis of sperm movement in mammals

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                Author and article information

                Zootecnia Tropical
                Zootecnia Trop.
                Instituto Nacional de Investigaciones Agricolas INIA, Maracay, Venezuela. (Maracay, Aragua, Venezuela )
                September 2006
                : 24
                : 3
                : 267-280
                [01] Maracay Aragua orgnameUniversidad Central de Venezuela orgdiv1Facultad de Ciencias Veterinarias marisabelalbers@yahoo.es
                S0798-72692006000300006 S0798-7269(06)02400306


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