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Abstract
Corticotropin-induced secreted protein (CISP) is a trimeric protein secreted by bovine
adrenocortical cells in response to ACTH, that is likely to represent the bovine form
of thrombospondin-2 (TSP2). This study was aimed at delineating the respective effects
of CISP/TSP2 and TSP1 (thrombospondin-1) on adrenocortical cell attachment and spreading.
TSP1 and CISP/TSP2 were found to slightly reduce the attachment of adrenocortical
cells to plastic in the presence of serum but exhibited a pronounced differential
effect on cell spreading. CISP/TSP2 inhibited adrenocortical cell spreading in a dose-dependent
manner (maximal effect with 40 micrograms/ml) whereas TSP1 (up to 100 micrograms/ml)
did not influence this process. The inhibition of spreading was observed whether plates
were coated with CISP/TSP2 alone or with a mixture of CISP/TSP2 and fibronectin. We
suggest that the inhibition of in vitro adrenocortical cell spreading by CISP/TSP2
is indicative of an implication of this protein in the migration of adrenocortical
cells in vivo.