In light of discovering the new antiplatelet from the natural source, this study was undertaken to determine the inhibitory effect of Rhodomyrtus tomentosa methanolic leaves extracts on P-selectin platelet aggregation of the ABO blood group. Whole blood samples from each blood group were collected tested with different concentration of leaves extract by using ELISA method. The inhibition was considered to occur if the values of concentration P selectin detected below 1.56 ng/ml. Instead of inhibiting the P-selectin expression as antiplatelet, Rhodomyrtus tomentosaaccelerates the P-selectin expression as an agonist in a dose-dependent manner. In conclusion, no antiplatelet effects were seen.