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      Energy expenditure of rugby players during a 14-day in-season period, measured using doubly labelled water

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          Abstract

          Criterion data for total energy expenditure (TEE) in elite rugby are lacking, which prediction equations may not reflect accurately. This study quantified TEE of 27 elite male rugby league (RL) and rugby union (RU) players (U16, U20, U24 age groups) during a 14-day in-season period using doubly labelled water (DLW). Measured TEE was also compared to estimated, using prediction equations. Resting metabolic rate (RMR) was measured using indirect calorimetry, and physical activity level (PAL) estimated (TEE:RMR). Differences in measured TEE were unclear by code and age (RL 4369 ± 979; RU 4365 ± 1122; U16, 4010 ± 744; U20, 4414 ± 688; U24, 4761 ± 1523 Kcal day − 1). Differences in PAL (overall mean 2.0 ± 0.4) were unclear. Very likely differences were observed in RMR by code (RL 2366 ± 296; RU 2123 ± 269 Kcal day − 1). Differences in relative RMR between U20 and U24 were very likely (U16, 27 ± 4; U20, 23 ± 3; U24, 26 ± 5 Kcal kg − 1 day − 1). Differences were observed between measured and estimated TEE, using Schofield, Cunningham and Harris–Benedict equations for U16 (187 ± 614, unclear; − 489 ± 564, likely and − 90 ± 579, unclear Kcal day − 1), U20 (− 449 ± 698, likely; − 785 ± 650, very likely and − 452 ± 684, likely Kcal day − 1) and U24 players (− 428 ± 1292; − 605 ± 1493 and − 461 ± 1314 Kcal day − 1, all unclear). Rugby players have high TEE, which should be acknowledged. Large inter-player variability in TEE was observed demonstrating heterogeneity within groups, thus published equations may not appropriately estimate TEE.

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          The Official Positions of the International Society for Clinical Densitometry: acquisition of dual-energy X-ray absorptiometry body composition and considerations regarding analysis and repeatability of measures.

          In preparation for the International Society for Clinical Densitometry Position Development Conference of 2013 in Tampa, Florida, Task Force 2 was created as 1 of 3 task forces in the area of body composition assessment by dual-energy X-ray absorptiometry (DXA). The assignment was to review the literature, summarize the relevant findings, and formulate positions covering (1) accuracy and precision assessment, (2) acquisition of DXA body composition measures in patients, and (3) considerations regarding analysis and repeatability of measures. There were 6 primary questions proposed to the task force by the International Society for Clinical Densitometry board and expert panel. Based on a series of systematic reviews, 14 new positions were developed, which are intended to augment and define good clinical practice in quantitative assessment of body composition by DXA.
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            Reference Values for Body Composition and Anthropometric Measurements in Athletes

            Background Despite the importance of body composition in athletes, reference sex- and sport-specific body composition data are lacking. We aim to develop reference values for body composition and anthropometric measurements in athletes. Methods Body weight and height were measured in 898 athletes (264 female, 634 male), anthropometric variables were assessed in 798 athletes (240 female and 558 male), and in 481 athletes (142 female and 339 male) with dual-energy X-ray absorptiometry (DXA). A total of 21 different sports were represented. Reference percentiles (5th, 25th, 50th, 75th, and 95th) were calculated for each measured value, stratified by sex and sport. Because sample sizes within a sport were often very low for some outcomes, the percentiles were estimated using a parametric, empirical Bayesian framework that allowed sharing information across sports. Results We derived sex- and sport-specific reference percentiles for the following DXA outcomes: total (whole body scan) and regional (subtotal, trunk, and appendicular) bone mineral content, bone mineral density, absolute and percentage fat mass, fat-free mass, and lean soft tissue. Additionally, we derived reference percentiles for height-normalized indexes by dividing fat mass, fat-free mass, and appendicular lean soft tissue by height squared. We also derived sex- and sport-specific reference percentiles for the following anthropometry outcomes: weight, height, body mass index, sum of skinfold thicknesses (7 skinfolds, appendicular skinfolds, trunk skinfolds, arm skinfolds, and leg skinfolds), circumferences (hip, arm, midthigh, calf, and abdominal circumferences), and muscle circumferences (arm, thigh, and calf muscle circumferences). Conclusions These reference percentiles will be a helpful tool for sports professionals, in both clinical and field settings, for body composition assessment in athletes.
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              Doubly labelled water assessment of energy expenditure: principle, practice, and promise

              The doubly labelled water method for the assessment of energy expenditure was first published in 1955, application in humans started in 1982, and it has become the gold standard for human energy requirement under daily living conditions. The method involves enriching the body water of a subject with heavy hydrogen (2H) and heavy oxygen (18O), and then determining the difference in washout kinetics between both isotopes, being a function of carbon dioxide production. In practice, subjects get a measured amount of doubly labelled water (2H2 18O) to increase background enrichment of body water for 18O of 2000 ppm with at least 180 ppm and background enrichment of body water for 2H of 150 ppm with 120 ppm. Subsequently, the difference between the apparent turnover rates of the hydrogen and oxygen of body water is assessed from blood-, saliva-, or urine samples, collected at the start and end of the observation interval of 1–3 weeks. Samples are analyzed for 18O and 2H with isotope ratio mass spectrometry. The doubly labelled water method is the indicated method to measure energy expenditure in any environment, especially with regard to activity energy expenditure, without interference with the behavior of the subjects. Applications include the assessment of energy requirement from total energy expenditure, validation of dietary assessment methods and validation of physical activity assessment methods with doubly labelled water measured energy expenditure as reference, and studies on body mass regulation with energy expenditure as a determinant of energy balance.
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                Author and article information

                Contributors
                01138124091 , D.R.Smith@leedsbeckett.ac.uk
                Journal
                Eur J Appl Physiol
                Eur. J. Appl. Physiol
                European Journal of Applied Physiology
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                1439-6319
                1439-6327
                20 January 2018
                20 January 2018
                2018
                : 118
                : 3
                : 647-656
                Affiliations
                [1 ]ISNI 0000 0001 0745 8880, GRID grid.10346.30, Institute for Sport, Physical Activity and Leisure, , Leeds Beckett University, ; G19 Fairfax Hall, Headingley Campus, Leeds, LS6 3QN UK
                [2 ]ISNI 0000 0000 9762 0345, GRID grid.224137.1, Stable Isotope Biochemistry Laboratory, , Scottish Universities Environmental Research Centre, ; East Kilbride, UK
                [3 ]Leeds Rhinos RLFC, Leeds, UK
                [4 ]Yorkshire Carnegie RUFC, Leeds, UK
                [5 ]The Rugby Football League, Leeds, UK
                Author notes

                Communicated by Jean-René Lacour.

                Author information
                http://orcid.org/0000-0001-5711-9609
                Article
                3804
                10.1007/s00421-018-3804-4
                5805815
                29353321
                390cbfdc-15cf-4e27-acde-4c5df5003b94
                © The Author(s) 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

                History
                : 28 September 2017
                : 11 January 2018
                Categories
                Original Article
                Custom metadata
                © Springer-Verlag GmbH Germany, part of Springer Nature 2018

                Anatomy & Physiology
                team sport,rugby,adolescent,athletes,metabolic rate,energy requirements,energy expenditure,doubly labelled water,physical activity level,nutrition

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