Epithelial-mesenchymal transition of absorptive enterocytes and depletion of Peyer's patch M cells after PEDV infection

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

This study focused on intestinal restitution including phenotype switching of absorptive enterocytes and the abundance of different enterocyte subtypes in weaned pigs after porcine epidemic diarrhea virus (PEDV) infection. At 10 days post-PEDV-inoculation, the ratio of villus height to crypt depth in both jejunum and ileum had restored, and the PEDV antigen was not detectable. However, enterocytes at the villus tips revealed epithelial-mesenchymal transition (EMT) in the jejunum in which E-cadherin expression decreased while expression of N-cadherin, vimentin, and Snail increased. Additionally, there was reduced expression of actin in microvilli and Zonula occludens-1 (ZO-1) in tight junctions. Moreover, the protein concentration of transforming growth factor β1 (TGFβ1), which mediates EMT and cytoskeleton alteration, was increased. We also found a decreased number of Peyer's patch M cells in the ileum. These results reveal incomplete restitution of enterocytes in the jejunum and potentially impaired immune surveillance in the ileum after PEDV infection.

Related collections

Most cited references 59

Record: found
Abstract: found
Article: not found

Molecular mechanisms of epithelial-mesenchymal transition.

Samy Lamouille, Jian Xu, Rik Derynck (2014)

The transdifferentiation of epithelial cells into motile mesenchymal cells, a process known as epithelial-mesenchymal transition (EMT), is integral in development, wound healing and stem cell behaviour, and contributes pathologically to fibrosis and cancer progression. This switch in cell differentiation and behaviour is mediated by key transcription factors, including SNAIL, zinc-finger E-box-binding (ZEB) and basic helix-loop-helix transcription factors, the functions of which are finely regulated at the transcriptional, translational and post-translational levels. The reprogramming of gene expression during EMT, as well as non-transcriptional changes, are initiated and controlled by signalling pathways that respond to extracellular cues. Among these, transforming growth factor-β (TGFβ) family signalling has a predominant role; however, the convergence of signalling pathways is essential for EMT.

0 comments Cited 1924 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: not found

Stem cells, self-renewal, and differentiation in the intestinal epithelium.

Laurens van der Flier, Hans Clevers (2009)

The mammalian intestine is covered by a single layer of epithelial cells that is renewed every 4-5 days. This high cell turnover makes it a very attractive and comprehensive adult organ system for the study of cell proliferation and differentiation. The intestine is composed of proliferative crypts, which contain intestinal stem cells, and villi, which contain differentiated specialized cell types. Through the recent identification of Lgr5, an intestinal stem cell marker, it is now possible to visualize stem cells and study their behavior and differentiation in a much broader context. In this review we describe the identification of intestinal stem cells. We also discuss genetic studies that have helped to elucidate those signals important for progenitor cells to differentiate into one of the specialized intestinal epithelial cell types. These studies describe a genetic hierarchy responsible for cell fate commitment in normal gut physiology. Where relevant we also mention aberrant deregulation of these molecular pathways that results in colon cancer.

0 comments Cited 332 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: not found

Complex networks orchestrate epithelial-mesenchymal transitions.

Jean Paul Thiery, Jonathan P Sleeman (2006)

Epithelial-mesenchymal transition is an indispensable mechanism during morphogenesis, as without mesenchymal cells, tissues and organs will never be formed. However, epithelial-cell plasticity, coupled to the transient or permanent formation of mesenchyme, goes far beyond the problem of cell-lineage segregation. Understanding how mesenchymal cells arise from an epithelial default status will also have a strong impact in unravelling the mechanisms that control fibrosis and cancer progression.

0 comments Cited 314 times – based on 0 reviews      Review now

All references

Author and article information

Journal

Journal ID (nlm-ta): Virology

Journal ID (iso-abbrev): Virology

Title: Virology

Publisher: Elsevier Inc.

ISSN (Print): 0042-6822

ISSN (Electronic): 1096-0341

Publication date PMC-release: 11 October 2020

Publication date (Print): 2 January 2021

Publication date (Electronic): 11 October 2020

Volume: 552

Pages: 43-51

Affiliations

[a ]Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA

[b ]Department of Animal Science, Iowa State University, College of Agriculture and Life Sciences, Ames, IA, USA

[c ]Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA

Author notes

[∗ ]Corresponding author. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine 1800 Christensen Drive, Ames, IA, USA, 50011-1134.

Article

Publisher Item ID: S0042-6822(20)30196-3

DOI: 10.1016/j.virol.2020.08.018

PMC ID: 7548064

PubMed ID: 33059319

SO-VID: 3935451a-7091-4737-9294-5759c3beba59

License:

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

History

Date received : 26 June 2020

Date revision received : 18 August 2020

Date accepted : 31 August 2020

Comments

Comment on this article

Cited by 5

See all cited by

Most referenced authors 907

See all reference authors

- Version 1
- Version 1