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      A note on the use of picric acid-paraformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunocytochemistry

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      Neuroscience
      Elsevier BV

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          Abstract

          The buffered picric acid paraformaldehyde fixative originally recommended for electronmicroscopy and which has since been used occasionally for light-microscopic immunocytochemistry, has been supplemented with glutaraldehyde and used as primary fixative for the perfusion of rat brains. In the basal ganglia and preoptic area, substance P, somatostatin and leu-enkephalin immunoreactive material was localized with the unlabelled antibody enzyme method in thick sections cut from freeze-thaw treated blocks. Good penetration of the antibodies without the use of detergents and the light background of the osmium-treated sections allowed the selection for electron-microscopy of immunoreactive structures as small as individual boutons that had been identified at the light-microscopic level. It is suggested that the procedure may be useful for electron-microscopic sampling of immunoreactive structures occurring infrequently over a large area or for the electron-microscopic study of light-microscopically classified neurons.

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          Author and article information

          Journal
          Neuroscience
          Neuroscience
          Elsevier BV
          03064522
          July 1982
          July 1982
          : 7
          : 7
          : 1779-1783
          Article
          10.1016/0306-4522(82)90035-5
          6181433
          3a209e4c-b4b9-4708-afc6-6937bc71e36e
          © 1982

          https://www.elsevier.com/tdm/userlicense/1.0/

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