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      Androgen metabolism assessment by routine gas chromatography/mass spectrometry profiling of plasma steroids: part 1, unconjugated steroids

      , , , ,
      Steroids
      Elsevier BV

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          Abstract

          Using gas chromatography/mass spectrometry we have developed a method for the simultaneous determination of six plasma steroids: testosterone, 4-androstenedione, 17 alpha-hydroxyprogesterone, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-dihydrotestosterone, and dehydroepiandrosterone. For each analyte, a deuterium-labeled internal standard was used for quantification. Due to the high isotopic purity of our standards, no complex corrections for isotope contributions were necessary. The procedure provides a sensitive and specific technique with good accuracy and precision.

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          Author and article information

          Journal
          Steroids
          Steroids
          Elsevier BV
          0039128X
          July 1992
          July 1992
          : 57
          : 7
          : 319-324
          Article
          10.1016/0039-128X(92)90050-J
          1412568
          3a57072f-fb67-4951-a98e-b9d998ab3c0d
          © 1992

          https://www.elsevier.com/tdm/userlicense/1.0/

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