5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Impact of PDGF‐BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post‐operation

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Current methods for tendon rupture repair suffer from two main drawbacks: insufficient strength and adhesion formation, which lead to rerupture and impaired gliding. A novel polymer tube may help to overcome these problems by allowing growth factor delivery to the wound site and adhesion reduction, and by acting as a physical barrier to the surrounding tissue. In this study, we used a bilayered DegraPol ® tube to deliver PDGF‐BB to the wound site in a full‐transection rabbit Achilles tendon model. We then performed histological and immunohistochemical analysis at 3 weeks postoperation. Sustained delivery of PDGF‐BB to the healing Achilles tendon led to a significantly more homogenous cell distribution within the healing tissue. Lower cell densities next to the implant material were determined for +PDGF‐BB samples compared to −PDGF‐BB. PDGF‐BB application increased proteoglycan content and reduced alpha‐SMA + areas, clusters of different sizes, mainly vessels. Finally, PDGF‐BB reduced collagens I and III in the extracellular matrix. The sustained delivery of PDGF‐BB via an electrospun DegraPol ® tube accelerated tendon wound healing by causing a more uniform cell distribution with higher proteoglycan content and less fibrotic tissue. Moreover, the application of this growth factor reduced collagen III and alpha‐SMA, indicating a faster and less fibrotic tendon healing.

          Abstract

          Sustained PDGF‐BB delivery via DegraPol ® implant to a fully transected rabbit Achilles tendon leads to a more homogenous cellular distribution in the healing tendon tissue in vivo. Increased proteoglycan content and reduced collagen III and alpha‐SMA + areas confirm the positive effect of PDGF‐BB.

          Related collections

          Most cited references32

          • Record: found
          • Abstract: found
          • Article: not found

          Tendon injury and tendinopathy: healing and repair.

          Tendon disorders are frequent and are responsible for substantial morbidity both in sports and in the workplace. Tendinopathy, as opposed to tendinitis or tendinosis, is the best generic descriptive term for the clinical conditions in and around tendons arising from overuse. Tendinopathy is a difficult problem requiring lengthy management, and patients often respond poorly to treatment. Preexisting degeneration has been implicated as a risk factor for acute tendon rupture. Several physical modalities have been developed to treat tendinopathy. There is limited and mixed high-level evidence to support the, albeit common, clinical use of these modalities. Further research and scientific evaluation are required before biological solutions become realistic options.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Vascular smooth muscle cell in atherosclerosis.

            Vascular smooth muscle cells (VSMCs) exhibit phenotypic and functional plasticity in order to respond to vascular injury. In case of the vessel damage, VSMCs are able to switch from the quiescent 'contractile' phenotype to the 'proinflammatory' phenotype. This change is accompanied by decrease in expression of smooth muscle (SM)-specific markers responsible for SM contraction and production of proinflammatory mediators that modulate induction of proliferation and chemotaxis. Indeed, activated VSMCs could efficiently proliferate and migrate contributing to the vascular wall repair. However, in chronic inflammation that occurs in atherosclerosis, arterial VSMCs become aberrantly regulated and this leads to increased VSMC dedifferentiation and extracellular matrix formation in plaque areas. Proatherosclerotic switch in VSMC phenotype is a complex and multistep mechanism that may be induced by a variety of proinflammatory stimuli and hemodynamic alterations. Disturbances in hemodynamic forces could initiate the proinflammatory switch in VSMC phenotype even in pre-clinical stages of atherosclerosis. Proinflammatory signals play a crucial role in further dedifferentiation of VSMCs in affected vessels and propagation of pathological vascular remodelling.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Novel Model of Tendon Regeneration Reveals Distinct Cell Mechanisms Underlying Regenerative and Fibrotic Tendon Healing

              To date, the cell and molecular mechanisms regulating tendon healing are poorly understood. Here, we establish a novel model of tendon regeneration using neonatal mice and show that neonates heal via formation of a ‘neo-tendon’ that differentiates along the tendon specific lineage with functional restoration of gait and mechanical properties. In contrast, adults heal via fibrovascular scar, aberrant differentiation toward cartilage and bone, with persistently impaired function. Lineage tracing identified intrinsic recruitment of Scx-lineage cells as a key cellular mechanism of neonatal healing that is absent in adults. Instead, adult Scx-lineage tenocytes are not recruited into the defect but transdifferentiate into ectopic cartilage; in the absence of tenogenic cells, extrinsic αSMA-expressing cells persist to form a permanent scar. Collectively, these results establish an exciting model of tendon regeneration and uncover a novel cellular mechanism underlying regenerative vs non-regenerative tendon healing.
                Bookmark

                Author and article information

                Contributors
                johanna.buschmann@usz.ch
                Journal
                FEBS Open Bio
                FEBS Open Bio
                10.1002/(ISSN)2211-5463
                FEB4
                FEBS Open Bio
                John Wiley and Sons Inc. (Hoboken )
                2211-5463
                05 February 2020
                March 2020
                : 10
                : 3 ( doiID: 10.1002/feb4.v10.3 )
                : 327-337
                Affiliations
                [ 1 ] Division of Plastic Surgery and Hand Surgery University Hospital Zurich Switzerland
                [ 2 ] Laboratory of Applied Mechanobiology ETH Zürich Switzerland
                [ 3 ] ab medica Cerro Maggiore Italy
                Author notes
                [*] [* ] Correspondence

                J. Buschmann, Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, ZKF, Sternwartstrasse 14, Zurich 8091, Switzerland

                Fax: +41 44 255 50 47

                Tel: +41 44 255 98 95

                E‐mail: johanna.buschmann@ 123456usz.ch

                Author information
                https://orcid.org/0000-0001-7919-7448
                Article
                FEB412736
                10.1002/2211-5463.12736
                7050259
                31571428
                3ad3dd1b-4c90-404b-8664-08fecb2b4e7e
                © 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

                This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                : 05 April 2019
                : 12 September 2019
                : 27 September 2019
                Page count
                Figures: 5, Tables: 0, Pages: 11, Words: 6307
                Funding
                Funded by: Hartmann‐Müller Foundation , open-funder-registry 10.13039/501100008475;
                Funded by: Wolfermann‐Nägeli Foundation
                Funded by: Fonds für Medizinische Forschung der Universität Zürich
                Funded by: ab medica, Italy
                Categories
                Research Article
                Research Articles
                Custom metadata
                2.0
                March 2020
                Converter:WILEY_ML3GV2_TO_JATSPMC version:5.7.6.1 mode:remove_FC converted:02.03.2020

                alpha‐sma,collagen,pdgf‐bb,proteoglycan,rabbit achilles tendon

                Comments

                Comment on this article