Blog
About

22
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      CLIP identifies Nova-regulated RNA networks in the brain.

      Science (New York, N.Y.)

      Ultraviolet Rays, 3' Untranslated Regions, Reverse Transcriptase Polymerase Chain Reaction, metabolism, RNA-Binding Proteins, genetics, RNA, Messenger, RNA Precursors, Precipitin Tests, Neurons, Neural Inhibition, Nerve Tissue Proteins, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 9, Mice, Membrane Proteins, Introns, Exons, Carrier Proteins, Brain, Antigens, Neoplasm, Animals, Alternative Splicing

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Nova proteins are neuron-specific antigens targeted in paraneoplastic opsoclonus myoclonus ataxia (POMA), an autoimmune neurologic disease characterized by abnormal motor inhibition. Nova proteins regulate neuronal pre-messenger RNA splicing by directly binding to RNA. To identify Nova RNA targets, we developed a method to purify protein-RNA complexes from mouse brain with the use of ultraviolet cross-linking and immunoprecipitation (CLIP).Thirty-four transcripts were identified multiple times by Nova CLIP.Three-quarters of these encode proteins that function at the neuronal synapse, and one-third are involved in neuronal inhibition.Splicing targets confirmed in Nova-/- mice include c-Jun N-terminal kinase 2, neogenin, and gephyrin; the latter encodes a protein that clusters inhibitory gamma-aminobutyric acid and glycine receptors, two previously identified Nova splicing targets.Thus, CLIP reveals that Nova coordinately regulates a biologically coherent set of RNAs encoding multiple components of the inhibitory synapse, an observation that may relate to the cause of abnormal motor inhibition in POMA.

          Related collections

          Author and article information

          Journal
          14615540
          10.1126/science.1090095

          Comments

          Comment on this article