Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Activation of the xylDLEGF promoter of the TOL toluene-xylene degradation pathway by overproduction of the xylS regulatory gene product.

Journal of Bacteriology

genetics, Cloning, Molecular, Escherichia coli, metabolism, Genes, Bacterial, Genes, Regulator, Plasmids, Promoter Regions, Genetic, Pseudomonas, Toluene, Xylenes, Bacterial Proteins, biosynthesis

Read this article at

ScienceOpenPMC
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      The xylS regulatory gene of the Pseudomonas putida TOL plasmid (pWWO) has been cloned under the transcriptional control of the Escherichia coli tac promoter in a broad-host-range controlled-expression vector. Induction with isopropylthiogalactoside allowed overproduction and characterization of the xylS product by specific interaction with the TOL meta-cleavage pathway operator-promoter region (OP2) in vivo in E. coli. Examination of plasmid-specified polypeptides in E. coli maxicells led to identification of the xylS product as a 36-kilodalton polypeptide. The operator sequences required for xylS interactions lay upstream of the OP2 transcriptional start, and the xylS gene product recognized this region even in the absence of known coinducers.

      Related collections

      Author and article information

      Journal
      3301806
      212435

      Comments

      Comment on this article