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Comparative Genomics Identifies Candidate Genes for Infectious Salmon Anemia (ISA) Resistance in Atlantic Salmon (Salmo salar)

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      Abstract

      Infectious salmon anemia (ISA) has been described as the hoof and mouth disease of salmon farming. ISA is caused by a lethal and highly communicable virus, which can have a major impact on salmon aquaculture, as demonstrated by an outbreak in Chile in 2007. A quantitative trait locus (QTL) for ISA resistance has been mapped to three microsatellite markers on linkage group (LG) 8 (Chr 15) on the Atlantic salmon genetic map. We identified bacterial artificial chromosome (BAC) clones and three fingerprint contigs from the Atlantic salmon physical map that contains these markers. We made use of the extensive BAC end sequence database to extend these contigs by chromosome walking and identified additional two markers in this region. The BAC end sequences were used to search for conserved synteny between this segment of LG8 and the fish genomes that have been sequenced. An examination of the genes in the syntenic segments of the tetraodon and medaka genomes identified candidates for association with ISA resistance in Atlantic salmon based on differential expression profiles from ISA challenges or on the putative biological functions of the proteins they encode. One gene in particular, HIV-EP2/MBP-2, caught our attention as it may influence the expression of several genes that have been implicated in the response to infection by infectious salmon anemia virus (ISAV). Therefore, we suggest that HIV-EP2/MBP-2 is a very strong candidate for the gene associated with the ISAV resistance QTL in Atlantic salmon and is worthy of further study.

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      The online version of this article (doi:10.1007/s10126-010-9284-0) contains supplementary material, which is available to authorized users.

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            Author and article information

            Affiliations
            [1 ]Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC Canada V5A 1S6
            [2 ]Department of Biology, University of Victoria, Victoria, BC Canada V8W 3N5
            Contributors
            +1-778-7825637 , +1-778-7825583 , wdavidso@sfu.ca
            Journal
            Mar Biotechnol (NY)
            Marine Biotechnology (New York, N.y.)
            Springer-Verlag (New York )
            1436-2228
            1436-2236
            16 April 2010
            16 April 2010
            April 2011
            : 13
            : 2
            : 232-241
            3084937
            20396924
            9284
            10.1007/s10126-010-9284-0
            © The Author(s) 2010
            Categories
            Original Article
            Custom metadata
            © Springer Science+Business Media, LLC 2011

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