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      Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

      1

      Reproduction in Domestic Animals

      Wiley

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          Storage of boar semen.

          The problems, aspects and methods of liquid storage and freeze-thawing of boar semen are discussed and a review is given on examination of spermatozoa by the recent fluorescent staining methods.
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            Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function

             PF Watson (1995)
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              Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders.

              Although glutathione content in boar spermatozoa has been previously reported, the effect of reduced glutathione (GSH) on semen parameters and the fertilizing ability of boar spermatozoa after cryopreservation has never been evaluated. In this study, GSH content was determined in ejaculated boar spermatozoa before and after cryopreservation. Semen samples were centrifuged and GSH content in the resulting pellet monitored spectrophotometrically. The fertilizing ability of frozen-thawed boar sperm was also tested in vitro by incubating sperm with in vitro matured oocytes obtained from gilts. GSH content in fresh semen was 3.84 +/- 0.21 nM GSH/10(8) sperm. Following semen cryopreservation, there was a 32% decrease in GSH content (P < 0.0001). There were significant differences in sperm GSH content between different boars and after various preservation protocols (P = 0.0102 ). The effect of addition of GSH to the freezing and thawing extenders was also evaluated. Addition of 5 mM GSH to the freezing extender did not have a significant effect on standard semen parameters or sperm fertilizing ability after thawing. In contrast, when GSH was added to the thawing extender, a dose-dependent tendency to increase in sperm fertilizing ability was observed, although no differences were observed in standard semen parameters. In summary, (i) there was a loss in GSH content after cryopreservation of boar semen; (ii) addition of GSH to the freezing extender did not result in any improvement in either standard semen parameters or sperm fertilizing ability; and (iii) addition of GSH to the thawing extender resulted in a significant increase in sperm fertilizing ability. Nevertheless, future studies must conclude if this is the case for all boars. Furthermore, since addition of GSH to the thawing extender did not result in an improvement in standard semen parameters, this suggests that during the thawing process, GSH prevents damage of a sperm property that is critical in the fertilization process but that is not measured in the routine semen analysis.
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                Author and article information

                Journal
                Reproduction in Domestic Animals
                Reprod Dom Anim
                Wiley
                09366768
                July 2015
                July 2015
                July 14 2015
                : 50
                : 71-79
                Affiliations
                [1 ]Nuffield Department of Obstetrics and Gynaecology; Level 3; Women's Centre; John Radcliffe Hospital; University of Oxford; Headington Oxford UK
                Article
                10.1111/rda.12569
                © 2015
                Product
                Self URI (article page): http://doi.wiley.com/10.1111/rda.12569

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