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Abstract
Brain-derived neurotrophic factor (BDNF) is known to activate proline-directed Ser/Thr
protein kinases and to enhance glutamatergic transmission via a Rab3a-dependent molecular
pathway. The identity of molecular targets in BDNF's action on Rab3a pathway, a synaptic
vesicle protein involved in vesicle trafficking and synaptic plasticity, is not fully
known. Here we demonstrate that BDNF enhances depolarization-evoked efflux of [(3)H]-glutamate
from nerve terminals isolated from the CA1 region of the hippocampus. BDNF also potentiated
hyperosmotic shock-evoked [(3)H]-glutamate efflux, indicating an effect on the size
of the readily releasable pool. This effect of BDNF was completely abolished in nerve
terminals derived from Rim1alphaKO (Rab3 interacting molecule 1alpha null mutant)
mice. Using in vitro phosphorylation assays we identified two novel phosphorylation
sites, Ser447 and Ser745 that were substrates for ERK2, a proline-directed kinase
known to be activated by BDNF. The pSer447 site was phosphorylated under resting conditions
in hippocampal CA1 nerve terminals and its phosphorylation was enhanced by BDNF treatment,
as indicated by the use of a pSer447-RIM1alpha antibody we have developed. Together
these findings identify RIM1alpha, a component of the Rab3a molecular pathway in mediating
presynaptic plasticity, as a necessary factor in BDNF's enhancement of [(3)H]-glutamate
efflux from hippocampal CA1 nerve terminals and indicate a possible role for RIM1alpha
phosphorylation in BDNF-dependent presynaptic plasticity.