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      An innovative method to obtain porous PLLA scaffolds with highly spherical and interconnected pores.

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          Abstract

          Scaffolding is an essential issue in tissue engineering and scaffolds should answer certain essential criteria: biocompatibility, high porosity, and important pore interconnectivity to facilitate cell migration and fluid diffusion. In this work, a modified solvent casting-particulate leaching out method is presented to produce scaffolds with spherical and interconnected pores. Sugar particles (200-300 microm and 300-500 microm) were poured through a horizontal Meker burner flame and collected below the flame. While crossing the high temperature zone, the particles melted and adopted a spherical shape. Spherical particles were compressed in plastic mold. Then, poly-L-lactic acid solution was cast in the sugar assembly. After solvent evaporation, the sugar was removed by immersing the structure into distilled water for 3 days. The obtained scaffolds presented highly spherical interconnected pores, with interconnection pathways from 10 to 100 mum. Pore interconnection was obtained without any additional step. Compression tests were carried out to evaluate the scaffold mechanical performances. Moreover, rabbit bone marrow mesenchymal stem cells were found to adhere and to proliferate in vitro in the scaffold over 21 days. This technique produced scaffold with highly spherical and interconnected pores without the use of additional organic solvents to leach out the porogen.

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          Author and article information

          Journal
          J. Biomed. Mater. Res. Part B Appl. Biomater.
          Journal of biomedical materials research. Part B, Applied biomaterials
          Wiley-Blackwell
          1552-4981
          1552-4973
          Jul 2008
          : 86
          : 1
          Affiliations
          [1 ] Group of Cell and Tissue Engineering, LEMTA, Nancy-Université, CNRS, 2 avenue de la forêt de Haye, 54 500 Vandoeuvre, France. cedryck.vaquette@ensem.inpl-nancy.fr
          Article
          10.1002/jbm.b.30982
          18098188
          3cfcb1b8-04bf-4352-9939-2da0a860374a
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