For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
47
views
39
references
 Top references
cited by
43
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      393
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.

          Related collections

          Most cited references39

          • Record: found
          • Abstract: found
          • Article: not found

          The biological and social phenomenon of Lyme disease.

          A Barbour, D N Fish (1993)
          Lyme disease, unknown in the United States two decades ago, is now the most common arthropod-borne disease in the country and has caused considerable morbidity in several suburban and rural areas. The emergence of this disease is in part the consequence of the reforestation of the northeastern United States and the rise in deer populations. Unfortunately, an accurate estimation of its importance to human and animal health has not been made because of difficulties in diagnosis and inadequate surveillance activities. Strategies for prevention of Lyme disease include vector control and vaccines.
          Article 0 comments Cited 158 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            The Lyme disease agent exploits a tick protein to infect the mammalian host.

            Nandhini Ramamoorthi, Sukanya Narasimhan, Utpal Pal … (2005)
            The Lyme disease agent, Borrelia burgdorferi, is maintained in a tick-mouse cycle. Here we show that B. burgdorferi usurps a tick salivary protein, Salp15 (ref. 3), to facilitate the infection of mice. The level of salp15 expression was selectively enhanced by the presence of B. burgdorferi in Ixodes scapularis, first indicating that spirochaetes might use Salp15 during transmission. Salp15 was then shown to adhere to the spirochaete, both in vitro and in vivo, and specifically interacted with B. burgdorferi outer surface protein C. The binding of Salp15 protected B. burgdorferi from antibody-mediated killing in vitro and provided spirochaetes with a marked advantage when they were inoculated into naive mice or animals previously infected with B. burgdorferi. Moreover, RNA interference-mediated repression of salp15 in I. scapularis drastically reduced the capacity of tick-borne spirochaetes to infect mice. These results show the capacity of a pathogen to use a secreted arthropod protein to help it colonize the mammalian host.
            Article 0 comments Cited 149 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              An annotated catalog of salivary gland transcripts from Ixodes scapularis ticks.

              Jose Ribeiro, Francisco J Alarcon-Chaidez, Ivo Francischetti … (2006)
              Over 8000 expressed sequence tags from six different salivary gland cDNA libraries from the tick Ixodes scapularis were analyzed. These libraries derive from feeding nymphs infected or not with the Lyme disease agent, Borrelia burgdorferi, from unfed adults, and from adults feeding on a rabbit for 6-12 h, 18-24 h, and 3-4 days. Comparisons of the several libraries led to identification of several significantly differentially expressed transcripts. Additionally, over 500 new predicted protein sequences are described, including several novel gene families unique to ticks; no function can be presently ascribed to most of these novel families. Among the housekeeping-associated transcripts, we highlight those enzymes associated with post translation modification of amino acids, particularly those forming sulfotyrosine, hydroxyproline, and carboxyl-glutamic acid. Results support the hypothesis that gene duplication, most possibly including genome duplications, is a major player in tick evolution.
              Article 0 comments Cited 134 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                : Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, USA )
                ISSN (Electronic): 1932-6203
                Publication date Collection: 2011
                Publication date (Electronic): 5 January 2011
                Volume: 6
                Issue: 1
                Electronic Location Identifier: e15926
                Affiliations
                [1 ]Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America
                [2 ]Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
                [3 ]Department of Experimental Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
                [4 ]Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
                [5 ]Departamento de Parasitologia, Universidade de São Paulo, São Paulo, Brazil
                [6 ]Department of Chemical and Biomolecular of Engineering, University of Tennessee, Knoxville, Tennessee, United States of America
                [7 ]Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands
                New York University School of Medicine, United States of America
                Author notes

                Conceived and designed the experiments: TJS SN SD CvV JCMM EF. Performed the experiments: TJS SN SD KD KB. Analyzed the data: TJS SN SD JWRH CvV KB JCMM APvD EF. Contributed reagents/materials/analysis tools: ETB JCMM TvdP. Wrote the paper: TJS SN EF.

                Article
                Publisher ID: PONE-D-10-01943
                DOI: 10.1371/journal.pone.0015926
                PMC ID: 3016337
                PubMed ID: 21246036
                SO-VID: 3d0052fa-61e7-4e42-8f4c-212e991d9e2f
                Copyright © Schuijt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
                History
                Date received : 6 September 2010
                Date accepted : 2 December 2010
                Page count
                Pages: 10
                Categories
                Subject: Research Article
                Subject: Biology
                Subject: Immunology
                Subject: Immune System
                Subject: Complement System
                Subject: Immunity
                Subject: Adaptive Immunity
                Subject: Humoral Immunity
                Subject: Immune Activation
                Subject: Immune Defense
                Subject: Immunizations
                Subject: Inflammation
                Subject: Innate Immunity
                Subject: Antigen Processing and Recognition
                Subject: Immune Cells
                Subject: Immune Response
                Subject: Immunoglobulins
                Subject: Immunologic Techniques
                Subject: Microbiology
                Subject: Proteomics
                Subject: Protein Interactions
                Subject: Sequence Analysis
                Subject: Sequencing

                ScienceOpen disciplines: Uncategorized
                Data availability:
                ScienceOpen disciplines: Uncategorized

                Comments

                Comment on this article

                scite_

                Similar content393

                See all similar

                Cited by40

                See all cited by

                Most referenced authors396

                See all reference authors