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      Improved multilaser/multiparameter flow cytometer for analysis and sorting of cells and particles

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      Review of Scientific Instruments
      AIP Publishing

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          Electronic separation of biological cells by volume.

          A device capable of separating biological cells (suspended in a conducting medium) according to volume has been developed. Cell volume is measured in a Coulter aperture, and the cells are subsequently isolated in droplets of the medium which are charged according to the sensed volume. The charged droplets then enter an electrostatic field and are deflected into a collection vessel. Mixtures of mouse and human erythrocytes and a large volume component of mouse lymphoma cells were separated successfully. In tests with Chinese hamster ovary cells essentially all cells survived separation and grew at their normal rate.
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            Two-color immunofluorescence using a fluorescence-activated cell sorter.

            A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.
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              High resolution dual laser flow cytometry.

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                Author and article information

                Journal
                Review of Scientific Instruments
                Review of Scientific Instruments
                AIP Publishing
                0034-6748
                1089-7623
                November 1991
                November 1991
                : 62
                : 11
                : 2751-2764
                Article
                10.1063/1.1142210
                3d1cfc2c-4f1b-4d19-94b8-a6cc7a266d0f
                © 1991
                History

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