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      Endothelium-lndependent Relaxations to Acetylcholine and A23187 in the Human Umbilical Artery


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          The effects of acetylcholine (ACh) and A23187 on ring preparations from the human umbilical artery (HUA) were investigated and compared with the rat aorta (RA). The results from the HUA demonstrate that: (1) At both high (pO<sub>2</sub> > 600 mm Hg) and low O<sub>2</sub> tension pO<sub>2</sub> < 55 mm Hg), ACh and A23187 relaxed precontracted rings in a concentration-dependent and endothelium-independent manner. Changes in pO<sub>2</sub> did not influence the responses of the HUA to either relaxant. (2) Relaxation responses of the HUA to either ACh or A23187 were insensitive to methylene blue (50 µ M) , L-nitro-arginine methyl ester (100 µ M) , indomethacin (10 µ M)and nordihydroguaiaretic acid (50 µ M) . Relaxations initiated by ACh were also atropine-resistant. (3) Meclofenamic acid (3 µ M) suppressed the relaxations to A23187, but not ACh. (4) Regardless of pO<sub>2</sub> superoxide dismutase (100 U/ml) potentiated the relaxant effects of ACh, whereas mannitol (60 mM) enhanced ACh-initiated relaxations at high but not low pO<sub>2</sub>. (5) Ouabain (30 n M), high potassium (HK<sup>+</sup>, 60 mM) and tetraethylammonium (20 m M) inhibited responses to ACh. (6) Na<sup>+</sup>-free physiological saline solution inhibited both relaxations and oscillations initiated by either ACh or A23187. (7) Both nitroglycerin and exogenous nitric oxide (NO) fully, and 8-bromoguanosine 3’,5’-cyclic monophosphate partially, relaxed the HUA, and LY83583 (10 µ M) reversed such relaxations. (8) In the RA, relaxation responses to ACh and A23187 were endothelium-dependent and sensitivity was reduced under high versus low pO<sub>2</sub> conditions. We conclude that in the HUA, unlike in the RA, ACh and A23187 mediate their responses via an endothelium- and NO-independent processes), perhaps involving the release of a muscle-derived relaxing factor. ACh-initiated relaxations are mediated by activation of Na<sup>+</sup>, K<sup>+</sup>-ATPase, and subsequent hyperpolarization via K<sup>+</sup> efflux, whereas A23187-mediated relaxations result from the synthesis of an indomethacin-resistant cyclooxygenase product.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          23 September 2008
          : 31
          : 2
          : 92-105
          Department of Pharmacology and Therapeutics, Faculty of Medicine, The Health Sciences Centre, The University of Calgary, Calgary, Canada
          159035 J Vasc Res 1994;31:92–105
          © 1994 S. Karger AG, Basel

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          Page count
          Pages: 14
          Research Paper


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