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      • Record: found
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      Fast and accurate identification of cryptic and sympatric mayfly species of the Baetis rhodani group

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          Abstract

          Objective

          Species of the Baetis rhodani group are among the most widespread mayflies of the Palearctic region. However, frequent occurrence of morphologically cryptic species complicates the identification of sympatric species. Here, we proposed and tested a method for the fast, accurate, and cost-effective assignment of a large number of individuals to their putative species, based on high resolution melting profiles of a standard mitochondrial gene fragment. We tested this method using a system of three recently identified cryptic species inhabiting the Tyrrhenian Islands (western Mediterranean basin).

          Results

          Highly species-specific high resolution melting profiles were obtained, allowing the unequivocal attribution of each individual to the respective species. This assay provides a convenient and easily customizable alternative to traditional barcoding approaches, provided that the mayfly taxa occurring within the geographic area of interest have been previously identified and their high resolution melting profiles assessed.

          Electronic supplementary material

          The online version of this article (10.1186/s13104-017-3115-6) contains supplementary material, which is available to authorized users.

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          Molecular Cloning : A Laboratory Manual

          Joseph Sambrook, E. F. Fritsch, Tom Maniatis (1989)
          Book 0 comments Cited 3409 times – based on 0 reviews
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            Biology of Mayflies

            J Brittain (1982)
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              Multiple lines of evidence for demographic and range expansion of a temperate species (Hyla sarda) during the last glaciation.

              Roberta Bisconti, Daniele Canestrelli, Paolo Colangelo … (2011)
              Many temperate species experienced demographic and range contractions in response to climatic changes during Pleistocene glaciations. In this study, we investigate the evolutionary history of the Tyrrhenian tree frog Hyla sarda, a species inhabiting the Corsica-Sardinia island system (Western Mediterranean basin). We used sequence analysis of two mitochondrial (overall 1229 bp) and three nuclear (overall 1692 bp) gene fragments to assess the phylogeography and demographic history of this species, and species distribution modelling (SDM) to predict its range variation over time. Phylogeographic, historical demographic and SDM analyses consistently indicate that H. sarda does not conform to the scenario generally expected for temperate species but rather underwent demographic and range expansion mostly during the last glacial phase. Palaeogeographic data and SDM analyses suggest that such expansion was driven by the glaciation-induced increase in lowland areas during marine regression. This unusual scenario suggests that at least some temperate species may not have suffered the adverse effects of glacial climate on their population size and range extent, owing to the mitigating effects of other glaciations-induced palaeoenvironmental changes. We discuss previous clues for the occurrence of such a scenario in other species and some possible challenges with its identification. Early phylogeographic literature suggested that responses to the Pleistocene glacial-interglacial cycles were expected to vary among species and regions. Our results point out that such variation may have been greater than previously thought. © 2011 Blackwell Publishing Ltd.
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                Author and article information

                Contributors
                Roberta Bisconti:
                ORCID: http://orcid.org/0000-0002-0600-7436
                +39-0761-357771 , bisconti@unitus.it
                Roberta Tenchini: r.tenchini@unitus.it
                Carlo Belfiore: c.belfiore@unitus.it
                Giuseppe Nascetti: nascetti@unitus.it
                Daniele Canestrelli: canestrelli@unitus.it
                Journal
                Journal ID (nlm-ta): BMC Res Notes
                Journal ID (iso-abbrev): BMC Res Notes
                Title: BMC Research Notes
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1756-0500
                Publication date (Electronic): 8 January 2018
                Publication date PMC-release: 8 January 2018
                Publication date Collection: 2018
                Volume: 11
                Electronic Location Identifier: 7
                Affiliations
                ISNI 0000 0001 2298 9743, GRID grid.12597.38, Dipartimento di Scienze Ecologiche e Biologiche, , Università degli Studi della Tuscia, ; Viale dell’Università s.n.c., 01100 Viterbo, Italy
                Author information
                http://orcid.org/0000-0002-0600-7436
                Article
                Publisher ID: 3115
                DOI: 10.1186/s13104-017-3115-6
                PMC ID: 5759225
                PubMed ID: 29310715
                SO-VID: 3d7936c5-0b25-46e7-9fbc-e70a0af646b2
                Copyright © © The Author(s) 2018
                License:

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                Date received : 1 November 2017
                Date accepted : 21 December 2017
                Funding
                Funded by: University of Tuscia
                Categories
                Subject: Research Note
                Custom metadata
                issue-copyright-statement © The Author(s) 2018

                ScienceOpen disciplines: Medicine
                Keywords: high resolution melting (hrm) curve,species identification,baetis rhodani,tyrrhenian islands,real time pcr
                Data availability:
                ScienceOpen disciplines: Medicine
                Keywords: high resolution melting (hrm) curve, species identification, baetis rhodani, tyrrhenian islands, real time pcr

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