98
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Synchronization of Plasmodium falciparum Erythrocytic Stages in Culture

      ,
      The Journal of Parasitology
      JSTOR

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single and multiple ring-form infections. At the same time, varying degrees of lysis of erythrocytes infected with the more mature stages of the parasite was evident. Approximately 95% of the parasites were in the ring stage of development at 48 and 96 hr after sorbitol treatment-likewise, a high percentage of trophozoite and schizont stages was observed at 24, 72, and 120 hr. D-Mannitol produced similar, selective, lytic effects.

          Related collections

          Author and article information

          Journal
          The Journal of Parasitology
          The Journal of Parasitology
          JSTOR
          00223395
          June 1979
          June 1979
          : 65
          : 3
          : 418
          Article
          10.2307/3280287
          383936
          3d8235c5-0d06-4827-bb3d-eb8da6777d32
          © 1979
          History

          Comments

          Comment on this article