Blog
About

90
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      An Nrf2/Small Maf Heterodimer Mediates the Induction of Phase II Detoxifying Enzyme Genes through Antioxidant Response Elements

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Related collections

          Most cited references 37

          • Record: found
          • Abstract: not found
          • Article: not found

          Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

           U K Laemmli (1970)
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Single step method of RNA isolation by acid guanidium thiocyanate phenol chloroform extraction. Anal

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells.

              Embryonal stem (ES) cell lines, established in culture from peri-implantation mouse blastocysts, can colonize both the somatic and germ-cell lineages of chimaeric mice following injection into host blastocysts. Recently, ES cells with multiple integrations of retroviral sequences have been used to introduce these sequences into the germ-line of chimaeric mice, demonstrating an alternative to the microinjection of fertilized eggs for the production of transgenic mice. However, the properties of ES cells raise a unique possibility: that of using the techniques of somatic cell genetics to select cells with genetic modifications such as recessive mutations, and of introducing these mutations into the mouse germ line. Here we report the realization of this possibility by the selection in vitro of variant ES cells deficient in hypoxanthine guanine phosphoribosyl transferase (HPRT; EC 2.4.2.8), their use to produce germline chimaeras resulting in female offspring heterozygous for HPRT-deficiency, and the generation of HPRT-deficient preimplantation embryos from these females. In human males, HPRT deficiency causes Lesch-Nyhan syndrome, which is characterized by mental retardation and self-mutilation.
                Bookmark

                Author and article information

                Journal
                Biochemical and Biophysical Research Communications
                Biochemical and Biophysical Research Communications
                Elsevier BV
                0006291X
                July 1997
                July 1997
                : 236
                : 2
                : 313-322
                10.1006/bbrc.1997.6943
                © 1997

                https://www.elsevier.com/tdm/userlicense/1.0/

                Comments

                Comment on this article