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Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

Author(s): Pedro L Sánchez Fernández, Manfred Reetz, Yosephine Gumulya, Smita Soni, J D Carballeira

Publication date: 2010-12-07

Journal: Chemical Communications (Cambridge, England)

Keywords: Bacillus subtilis, enzymology, Directed Molecular Evolution, methods, Enzyme Stability, drug effects, genetics, Lipase, chemistry, Mutagenesis, Organic Chemicals, pharmacology, Solvents

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Abstract

Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.

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PubMed ID:: 20957255

DOI:: 10.1039/c0cc02657c

ScienceOpen disciplines: Chemistry

Keywords: Bacillus subtilis,enzymology,Directed Molecular Evolution,methods,Enzyme Stability,drug effects,genetics,Lipase,chemistry,Mutagenesis,Organic Chemicals,pharmacology,Solvents

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ScienceOpen disciplines: Chemistry

Keywords: Bacillus subtilis, enzymology, Directed Molecular Evolution, methods, Enzyme Stability, drug effects, genetics, Lipase, chemistry, Mutagenesis, Organic Chemicals, pharmacology, Solvents

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

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