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      Parathyroid Hormone Enhances Gentamicin Uptake by Opposum Kidney Cells but Not by LLC-PK1 Cells

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          Abstract

          Parathyroidectomy is proposed to be protective against gentamicin nephrotoxicity in rats. In the present study, we evaluated the effects of bovine PTH(1-34) on gentamicin accumulation in cultured kidney epithelial cell lines, oppossum kidney (OK) cells which possess PTH receptors and LLC-PK1 cells which are devoid of PTH receptors. Ten days after seeding, the culture medium was exchanged for medium containing 1 m M gentamicin and bovine PTH. The cell gentamicin concentration was measured by a substrate-labeled fluorescence immunoassay (TDA gentamicin kit). Gentamicin uptake was accelerated by bovine PTH in OK cells but not in LLC-PK1 cells. The enhancing effect of bovine PTH seems to be mediated by a cAMP-dependent process. The results suggest that PTH accelerates gentamicin accumulation in renal tissues and potentiates gentamicin nephrotoxicity.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1991
          1991
          11 December 2008
          : 58
          : 1
          : 85-89
          Affiliations
          First Department of Medicine, Osaka University Medical School, Osaka, Japan
          Article
          186383 Nephron 1991;58:85–89
          10.1159/000186383
          1649976
          © 1991 S. Karger AG, Basel

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          Pages: 5
          Categories
          Original Paper

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