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      Case Report: A Retrospective Serological Analysis Indicating Human Exposure to Tick-Borne Relapsing Fever Spirochetes in Texas

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          Abstract

          Presentation of Case In June 2013, a Caucasian male from Kerr county, Texas, with an extensive history of outdoor activity working with sheep, goats, and exotic game became ill, displaying fever, chills, uveitis, headache, retrobulbar pain, severe malaise, and weakness. Myalgia was centered on upper extremities, most notably the shoulders, arms, and hands, and by July 2013 the patient had experienced two febrile episodes (39°C). Numerous insect bites were reported, most notably on areas of the body that were in direct contact with the ground, but offending arthropods were not found. The patient was initially tested for Coxiella burnetti exposure, but repeated serological results demonstrated that phase I and phase II immunoglobulin G (IgG) antibody titers failed to increase. Antibiotic treatment was administered, and symptoms improved. A retrospective evaluation of the patient’s history and clinical summary led to suspicion of tick-borne relapsing fever borreliosis. The patient did not have a history of traveling outside of Texas prior to the onset of symptoms, and Borrelia turicatae was suspected as the causative agent. A serum sample was sent to us four months after antibiotic treatment, and we implemented a molecular approach to determine seroreactivity against two diagnostic antigens for relapsing fever spirochetes, recombinant glycerophosphodiester phosphodiesterase (rGlpQ) and Borrelia immunogenic protein A (rBipA). Approach Ethics Statement Verbal and written informed consent was obtained from the patient to test the serum sample. The study was submitted to the Mississippi State University Institutional Review Board, protocol #13–369, and approved by designated review. Protein Analysis, Production of Diagnostic Antigens, and Serological Testing rGlpQ has been used to evaluate mammalian exposure to species distributed in the western United States and East and West Africa [1–4]. The gene is absent from other pathogenic spirochetes, and Schwan and colleagues first characterized rGlpQ as an antigen that could discriminate between exposure to relapsing fever and Lyme-causing Borrelia [1]. Amino acid alignments of GlpQ using Vector NTI 11.5 software (Life Technologies, Foster City, California, US) indicated the B. turicatae homologue is highly conserved between New (B. turicatae, Borrelia parkeri, and Borrelia hermsii) and Old (Borrelia recurrentis) World species of relapsing fever spirochetes (Table 1). 10.1371/journal.pntd.0003617.t001 Table 1 Percent amino acid identity of GlpQ between species of relapsing fever spirochete. B. turicatae B. parkeri B. hermsii B. recurrentis B. turicatae - B. parkeri 97 - B. hermsii 87 87 - B. recurrentis 81 80 80 - To produce B. turicatae GlpQ as a recombinant fusion protein, the gene was amplified using genomic DNA from the 91E135 isolate with 5ʹ-CACCATGAAATTAATTAAAACAA AATTATTAAT GCTTACAATGAATATTTTT-3ʹ and 5ʹ-TTGTTTTACAAACTTCACTAC TGTATCA GTAAAATCTGTAAAT-3ʹ forward and reverse primers, respectively. The amplicon was cloned into the pET102 expression vector, and the absence of nucleotide errors was confirmed by sequencing analysis using Vector NTI Advanced 11.5 software (Life Technologies). rGlpQ was produced as a six-histidine and thioredoxin fusion protein and purified by immobilized metal affinity chromatography using HisTrap FF Crude columns precharged with Ni2+ (GE Healthcare Life Sciences, Pittsburgh, Pennsylvania, US). Thioredoxin was removed by incubating the purified protein with 0.01 U of EKMax Enterokinase (Life Technologies), following the manufacturer’s protocol. The molecular mass of native GlpQ and rGlpQ are 40 and 45 kDa, respectively. Previous studies reported the identification and antigenicity of BipA and indicated a homologue was absent outside of relapsing fever Borrelia [5,6]. BipA also shares 24%–76% amino acid identity between relapsing fever spirochete homologues (Table 2) and was demonstrated to differentiate between infections caused by B. turicatae and B. hermsii [5]. B. turicatae rBipA was produced using the pET102 expression system as previously described [5], and thioredoxin was left attached to maintain protein solubility. The molecular mass of native and rBipA are 60 and 75 kDa, respectively. 10.1371/journal.pntd.0003617.t002 Table 2 Percent amino acid identity of BipA between species of relapsing fever spirochete. B. turicatae B. parkeri B. hermsii B. recurrentis B. turicatae - B. parkeri 76 - B. hermsii 36 34 - B. recurrentis 25 24 26 - Protein lysates from 1 x 107 spirochetes and 1 μg of rGlpQ and rBipA were electrophoretically separated and transferred to nitrocellulose membranes as previously described [5] using TGX gels, Mini-PROTEAN Tetra cell, and the Mini Trans Blot system (BioRad, Hercules, California, US). Immunoblots were probed with the patient’s serum sample at a 1:400 dilution, and Rec-Protein G-HRP (Life Technologies) diluted 1:4,000 was used as the secondary molecule. Serological reactivity to multiple proteins in the B. turicatae whole spirochete lysates, rGlpQ, and rBipA (Fig. 1A) was detected by chemiluminescence and indicated exposure to relapsing fever spirochetes. A serum sample from a subject living in a nonendemic region of the US without a history of exposure to relapsing fever spirochetes was used as a negative control (Fig. 1B). The nitrocellulose membrane was subsequently probed with a Monoclonal Anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich, St. Louis, Missouri, US), indicating the presence of the recombinant proteins (Fig. 1C and D). Additionally, the patient’s antibody titers to rGlpQ and rBipA were over a 1:6,400 dilution. 10.1371/journal.pntd.0003617.g001 Fig 1 Immunoblotting to evaluate antibody binding to B. turicatae protein lysates, rGlpQ, and rBipA (A). The asterisk and arrowhead represent the molecular masses for native GlpQ and BipA, respectively (A). rBipA was produced as a thioredoxin fusion protein and is 15 kDa larger than the native protein. Immunoblots were also probed with a serum sample from an uninfected patient (B) and a monoclonal antibody against the six-histidine fusion tag (C and D). Molecular masses are shown to the left of each immunoblot. Case Discussion The ecological overlap and nonspecific symptoms caused by vector-borne bacterial, viral, and parasitic pathogens signifies the importance of utilizing improved molecular assays to accurately determine pathogen exposure. In this report, the patient’s extensive outdoor activity and nonspecific clinical manifestation made it difficult to initially determine the causative agent. Retrospective analysis of serum reactivity to rGlpQ and rBipA suggested likely exposure to B. turicatae. A potential limitation of BipA as a species-specific antigen is that B. turicatae and B. parkeri are closely related [7], and the homologues share 76% amino-acid identity. However, we are unaware of a report indicating the occurrence of Ornithodoros parkeri, the vector for B. parkeri, in Texas, as the ticks have been collected throughout the western and midwestern US [8,9], further causing us to suspect B. turicatae. Accurate epidemiological studies for tick-borne relapsing fever borreliosis in Texas have been challenging because of nonspecific symptoms and limited molecular diagnostic assays. Human exposure has been evaluated retrospectively, by confirming spirochete colonization of the tick vector, Ornithodoros turicata, collected from presumed contact sites, which frequently included caves and manmade dugouts [10,11]. The disease has also been misdiagnosed as Lyme borreliosis because of similar neurological symptoms [10]. Further complicating a correct diagnosis was serological cross-reactivity with immunofluorescent assays (IFA) and enzyme-linked immunosorbent assays (ELISA), in which patient serum samples were assessed for reactivity to fixed Borrelia burgdorferi or total protein lysates, respectively [10]. Given the number of conserved antigens between Borrelia species and observed serological cross-reactivity [12,13], IFA and ELISA may be misleading. Furthermore, Ixodid ticks that transmit B. burgdorferi and feed for 5–7 days were not found to be attached on the patients [10]. Transmission of B. turicatae from the tick and the ecology and feeding behavior of O. turicata indicate that outdoor enthusiasts, military ground personnel, and low-income families living in primitive housing conditions are at-risk populations [14,15]. The ticks complete their blood meal within 5–60 minutes and subsequently return to the cave crevice, nest, or den in which they cohabit with small mammals [10,16]. Consequently, O. turicata is rarely found on the host, and a full blood meal is not required for transmission and infection [14]. Moreover, mammalian hosts supporting the maintenance of B. turicatae in nature are not completely known. Schwan and colleagues isolated the spirochetes from symptomatic domestic dogs, while the tick vector has been collected in caves [10,17,18]. These findings suggest a role of wild canids and bats in B. turicatae maintenance. Our ecological studies in Texas utilizing rGlpQ and rBipA to determine small mammal exposure to B. turicatae indicate coyotes and rodents may maintain the pathogens (manuscript in preparation). As improved molecular assays are utilized to evaluate mammalian exposure to relapsing fever spirochetes, we will further define the ecology and human health burden in regions where the pathogens are overlooked. Key Learning Points Given nonspecific clinical symptoms, relapsing fever spirochetes are likely underdiagnosed. Serological responses to rGlpQ and rBipA can indicate exposure to relapsing fever spirochetes. Likely at-risk populations include outdoor enthusiasts, military ground personnel, and those living in primitive housing conditions.

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          Most cited references 14

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          GlpQ: an antigen for serological discrimination between relapsing fever and Lyme borreliosis.

          Tick-borne relapsing fever is caused by numerous Borrelia species maintained in nature by Ornithodoros tick-mammal cycles. Serological confirmation is based on either an immunofluorescence assay or an enzyme-linked immunosorbent assay using whole cells or sonicated Borrelia hermsii as the antigen. However, antigenic variability of this bacterium's outer surface proteins and antigens shared with the Lyme disease spirochete (B. burgdorferi), may cause both false-negative and false-positive results when testing sera of patients suspected to have either relapsing fever or Lyme disease. To develop a specific serological test for relapsing fever, we created a genomic DNA library of B. hermsii, screened transformed Escherichia coli cells for immunoreactivity with high-titered (> or = 1:2,048) human anti-B. hermsii antiserum, and selected an immunoreactive clone (pSPR75) expressing a 39-kDa protein. DNA sequencing, subcloning, and serum adsorption experiments identified the immunoreactive protein as a homolog of GlpQ, a glycerophosphodiester phosphodiesterase identified previously in E. coli, Haemophilus influenzae, and Bacillus subtilis. Serum samples from humans and mice infected with B. hermsii or other species of relapsing fever spirochetes contained antibodies recognizing GlpQ, whereas serum samples from Lyme disease and syphilis patients were nonreactive. Serologic tests based on this antigen will identify people exposed previously to relapsing fever spirochetes and help clarify the distribution of relapsing fever and Lyme disease in situations in which the occurrence of their causative agents is uncertain.
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            Phylogenetic analysis of the spirochetes Borrelia parkeri and Borrelia turicatae and the potential for tick-borne relapsing fever in Florida.

            Isolates of Borrelia turicatae, Borrelia parkeri, and the Florida canine borrelia (FCB) were examined to further phylogenetically characterize the identities of these spirochetes in the United States. DNA sequences of four chromosomal loci (the 16S rRNA gene, flaB, gyrB, and glpQ) were determined for eight isolates of B. turicatae and six isolates of B. parkeri, which grouped the spirochetes into two distinct but closely related taxa (>98% sequence identity) separate from Borrelia hermsii. The FCB was clearly separated with the group identified as B. turicatae, confirming this bacterium as a relapsing fever spirochete. Therefore, the potential for tick-borne relapsing fever in humans and other animals exists in Florida and future efforts are needed to determine the enzootic hosts and distribution of this spirochete in the southeastern United States. Analysis of plasmids demonstrated both linear and circular forms in B. turicatae but only linear plasmids in B. parkeri, which should be of interest to investigators concerned with plasmid diversity and evolution within this group of spirochetes.
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              • Article: not found

              Cross-reactivity in serological tests for Lyme disease and other spirochetal infections.

              Serum specimens from 163 persons with Lyme disease, tick-borne or louse-borne relapsing fever, yaws, syphilis, leptospirosis, or Rocky Mountain spotted fever were analyzed to assess the specificity of indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and microscopic agglutination (MA) procedures. Strong cross-reactivity occurred when sera from individuals with Lyme disease, tick-borne relapsing fever, and louse-borne relapsing fever were tested against heterologous Borrelia antigens. Antibodies to Borrelia burgdorferi bound to Treponema pallidum in immunofluorescence tests for syphilis. Sera from subjects with syphilis cross-reacted in IFA tests and the ELISA for Lyme disease. Immunoglobulin antibodies to Borrelia or Treponema spirochetes, however, did not react with serovars of Leptospira interrogans in MA or IFA tests, and the prevalence of false-positive results in the reciprocal analyses was negligible.
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                Author and article information

                Contributors
                Role: Editor
                Journal
                PLoS Negl Trop Dis
                PLoS Negl Trop Dis
                plos
                plosntds
                PLoS Neglected Tropical Diseases
                Public Library of Science (San Francisco, CA USA )
                1935-2727
                1935-2735
                9 April 2015
                April 2015
                : 9
                : 4
                Affiliations
                [1 ]Department of Pediatrics, Section of Tropical Medicine, Baylor College of Medicine and Texas Children’s Hospital, Houston, Texas, United States of America
                [2 ]Texas State Guard, Medical Brigade, Uvalde, Texas, United States of America
                [3 ]Department of Biological Sciences, Mississippi State University, Starkville, Mississippi, United States of America
                [4 ]Department of Chemistry, Mississippi State University, Starkville, Mississippi, United States of America
                University of California San Diego School of Medicine, UNITED STATES
                Author notes

                The authors have declared that no competing interests exist.

                Article
                PNTD-D-14-02239
                10.1371/journal.pntd.0003617
                4391787
                25856342

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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                Figures: 1, Tables: 2, Pages: 5
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                Funding
                This study was supported by US Air Force School of Aerospace Medicine contract FA8650-12-D-6280. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Symposium

                Infectious disease & Microbiology

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