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      Color pattern variation in Trichomycterus iheringi (Eigenmann, 1917) (Siluriformes: Trichomycteridae) from rio Itatinga and rio Claro, São Paulo, Brazil

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          Abstract

          Color pattern is recognized as an important characteristic for diagnosing Trichomycterus species and for elucidating their relationships. An analysis based on morphological and molecular data confirms the existence of a single species of Trichomycterus in the rio Itatinga, a costal river drainage on the escarpment of the Serra do Mar and the rio Claro on the upper course of the rio Tietê. The only species found, Trichomycterus iheringi, shows two clearly distinct patterns of body pigmentation and intermediate color patterns related to body size and microhabitat preference.

          Translated abstract

          O padrão de coloração é reconhecido como uma característica importante para a diagnose das espécies do gênero Trichomycterus e no reconhecimento de suas relações de parentesco. A análise baseada em dados morfológicos e moleculares confirma a existência de uma única espécie de Trichomycterus no rio Itatinga, drenagem litorânea da Serra do Mar e no rio Claro, curso superior do rio Tietê. A única espécie do gênero encontrada, Trichomycterus iheringi, apresenta dois padrões de pigmentação do corpo bastante distintos e padrões de coloração intermediários relacionados ao tamanho corporal e ao micro-habitat.

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          Most cited references33

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          BioEdit - a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT

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            Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species.

            With millions of species and their life-stage transformations, the animal kingdom provides a challenging target for taxonomy. Recent work has suggested that a DNA-based identification system, founded on the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), can aid the resolution of this diversity. While past work has validated the ability of COI sequences to diagnose species in certain taxonomic groups, the present study extends these analyses across the animal kingdom. The results indicate that sequence divergences at COI regularly enable the discrimination of closely allied species in all animal phyla except the Cnidaria. This success in species diagnosis reflects both the high rates of sequence change at COI in most animal groups and constraints on intraspecific mitochondrial DNA divergence arising, at least in part, through selective sweeps mediated via interactions with the nuclear genome.
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              DNA barcoding Australia's fish species.

              Two hundred and seven species of fish, mostly Australian marine fish, were sequenced (barcoded) for a 655 bp region of the mitochondrial cytochrome oxidase subunit I gene (cox1). Most species were represented by multiple specimens, and 754 sequences were generated. The GC content of the 143 species of teleosts was higher than the 61 species of sharks and rays (47.1% versus 42.2%), largely due to a higher GC content of codon position 3 in the former (41.1% versus 29.9%). Rays had higher GC than sharks (44.7% versus 41.0%), again largely due to higher GC in the 3rd codon position in the former (36.3% versus 26.8%). Average within-species, genus, family, order and class Kimura two parameter (K2P) distances were 0.39%, 9.93%, 15.46%, 22.18% and 23.27%, respectively. All species could be differentiated by their cox1 sequence, although single individuals of each of two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data. In the neighbour-joining tree for all 754 sequences, four major clusters were apparent: chimaerids, rays, sharks and teleosts. Species within genera invariably clustered, and generally so did genera within families. Three taxonomic groups-dogfishes of the genus Squalus, flatheads of the family Platycephalidae, and tunas of the genus Thunnus-were examined more closely. The clades revealed after bootstrapping generally corresponded well with expectations. Individuals from operational taxonomic units designated as Squalus species B through F formed individual clades, supporting morphological evidence for each of these being separate species. We conclude that cox1 sequencing, or 'barcoding', can be used to identify fish species.
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                Author and article information

                Journal
                ni
                Neotropical Ichthyology
                Neotrop. ichthyol.
                Sociedade Brasileira de Ictiologia (Maringá, PR, Brazil )
                1679-6225
                1982-0224
                March 2010
                : 8
                : 1
                : 49-56
                Affiliations
                [01] Mogi das Cruzes SP orgnameUniversidade de Mogi das Cruzes orgdiv1Núcleo Integrado de Biotecnologia orgdiv2Laboratório de Genética de Organismos Aquáticos e Aquicultura Brazil
                [02] São José do Rio Preto SP orgnameUniversidade Estadual Paulista orgdiv1Departamento de Zoologia e Botânica orgdiv2Laboratório de Ictiologia Brazil
                Article
                S1679-62252010000100007 S1679-6225(10)00800107
                10.1590/S1679-62252010000100007
                3f60a30e-5689-4cf2-9225-9a1e9a894b9c

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 31 March 2010
                : 05 January 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 33, Pages: 8
                Product

                SciELO Brazil


                Cytocrome c oxidase,Body pigmentation,Southeast Brazil,Conspecificity

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